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The antigen processing\u2010defective cell line RMA\u2010S (H\u20102<jats:sup>b<\/jats:sup>) has a premature stop in the Tap 2 gene and probably therefore fails to deliver peptides into the ER, which leads to a low level of cell surface MHC class I molecules. Transfection of a Tap 2 gene restores to RMA\u2010S both MHC class I molecule expression and the ability to present influenza viral antigens. We investigated the ability of RMA\u2010S cells transfected with a Tap 2 gene to process and present alloantigens, Sendai and Rauscher viral antigens to allogeneic and virus\u2010specific cytotoxic T lymphocytes. We found that allogeneic peptides as well as Rauscher and Sendai viral peptides can be processed and presented by RMA\u2010S but at reduced levels. Transfection of a Tap 2 gene of mouse (BALB\/c, H\u20102<jats:sup>d<\/jats:sup>) or rat origin into RMA\u2010S increased the presentation of Sendai viral antigens and partially restored the presentation of allogeneic antigens. The already low level of Rauscher viral peptides presented by RMA\u2010S is not elevated by transfection of either Tap 2 gene into RMA\u2010S. This indicates a differential effect of transfection of a Tap 2 gene of rat or allogeneic mouse origin into RMA\u2010S on viral antigen processing.<\/jats:p>","DOI":"10.1002\/eji.1830231206","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T18:30:22Z","timestamp":1172773822000},"page":"3082-3088","source":"Crossref","is-referenced-by-count":7,"title":["Differential effect of transporter Tap 2 gene introduction into RMA\u2010S cells on viral antigen processing"],"prefix":"10.1002","volume":"23","author":[{"given":"Miriam A.","family":"Ossevoort","sequence":"first","affiliation":[]},{"given":"Alice J. A. M.","family":"Sijts","sequence":"additional","affiliation":[]},{"given":"Karin J. 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