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The binding of an anti\u2010WC1 monoclonal antibody (mAb) (SC\u201029) induces reversible growth arrest in proliferating interleukin (IL)\u20102\u2010dependent \u03b3\u03b4 T lymphocytes and this study has examined the relevant biochemical mechanisms. WC1 binding activates multiple protein tyrosine phosphatases causing specific tyrosine dephosphorylation in the absence of calcium mobilization. One of the dephosphorylated proteins was identified as the MAP kinase erk2. Another phosphotyrosine protein of 70 kDa, found to coprecipitate with p85 phosphoinositol (PI)3\u2010kinase was either dephosphorylated or uncoupled from the p85 PI 3\u2010kinase immunoprecipitate after WC1 receptor binding by mAb SC\u201029. The anti\u2010WC1\u2010induced tyrosine dephosphorylation was reversed by stimulation of \u03b3\u03b4 T cells with concanavalin A or anti\u2010CD3 mAb, demonstrating that at the biochemical level, mitogen stimulation is dominant to the growth\u2010arresting effects of anti\u2010WC1. It is therefore proposed that the activation of tyrosine phosphatases by WC1 binding and the resultant dephosphorylation of certain key signaling protein such as erk2 correlates with and may cause the induction of growth arrest in IL\u20102\u2010dependent \u03b3\u03b4 T cells, without affecting the cells ability to respond to antigen. Possible mechanisms, which include the inhibition of IL\u20102 signal transduction pathways, are discussed.<\/jats:p>","DOI":"10.1002\/eji.1830270321","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T04:32:52Z","timestamp":1172809972000},"page":"717-725","source":"Crossref","is-referenced-by-count":25,"title":["Growth arrest of \u03b3\u03b4 T cells induced by monoclonal antibody against WC1 correlates with activation of multiple tyrosine phosphatases and dephosphorylation of MAP kinase erk2"],"prefix":"10.1002","volume":"27","author":[{"given":"Paul A.","family":"Kirkham","sequence":"first","affiliation":[]},{"given":"Haru\u2010Hisa","family":"Takamatsu","sequence":"additional","affiliation":[]},{"given":"R. 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