{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T17:29:39Z","timestamp":1774114179415,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T00:00:00Z","timestamp":1322438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The T lineage glycoprotein CD6 is generally considered to be a costimulator of T\u2010cell activation. Here, we demonstrate that CD6 significantly reduces early and late T\u2010cell responses upon superantigen stimulation or TCR triggering by Abs. Measuring calcium mobilization in single cells responding to superantigen, we found that human T cells expressing rat CD6 react significantly less well compared with T cells not expressing the exogenous receptor. When the cytoplasmic domain of rat CD6 was removed, calcium responses were recovered, indicating that the inhibitory properties of CD6 are attributable to its cytoplasmic domain. Calcium responses, and also late indicators of T\u2010cell activation such as IL\u20102 release, were also diminished in TCR\u2010activated Jurkat cells expressing human CD6, compared with CD6\u2010deficient cells or cells expressing a cytoplasmic deletion mutant of human CD6. Similarly, calcium signals triggered by anti\u2010CD3 were enhanced in human T lymphocytes following morpholino\u2010mediated suppression of CD6 expression. Finally, the proliferation of T lymphocytes was increased when the CD6\u2013CD166 interaction was blocked with anti\u2010CD166 Abs, but inhibited when anti\u2010CD6 Abs were used. Our data suggest that CD6 is a signaling attenuator whose expression alone, i.e. in the absence of ligand engagement, is sufficient to restrain signaling in T cells.<\/jats:p>","DOI":"10.1002\/eji.201040528","type":"journal-article","created":{"date-parts":[[2011,9,28]],"date-time":"2011-09-28T13:50:00Z","timestamp":1317217800000},"page":"195-205","source":"Crossref","is-referenced-by-count":72,"title":["CD6 attenuates early and late signaling events, setting thresholds for T\u2010cell activation"],"prefix":"10.1002","volume":"42","author":[{"given":"Marta I.","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"Carine M.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]},{"given":"Mafalda","family":"Pinto","sequence":"additional","affiliation":[]},{"given":"St\u00e9phanie","family":"Fabre","sequence":"additional","affiliation":[]},{"given":"Ana Mafalda","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Simon F.","family":"Lee","sequence":"additional","affiliation":[]},{"given":"M\u00f3nica A. 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