{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:06:24Z","timestamp":1777889184212,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:00:00Z","timestamp":1339459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Effective CD<\/jats:styled-content>8+<\/jats:sup> T<\/jats:styled-content>\u2010cell responses against tumor or microbial antigens that are not directly expressed in antigen\u2010presenting cells (APC<\/jats:styled-content>s) depend on the cross\u2010presentation of these antigens on MHC c<\/jats:styled-content>lass I in APC<\/jats:styled-content>s. To identify signaling molecules that regulate cross\u2010presentation, we used lentiviral\u2010based RNA<\/jats:styled-content> interference to test the roles of hundreds of kinases and phosphatases in this process. Our study uncovered eight previously unknown genes, consisting of one positive and seven negative regulators of antigen cross\u2010presentation. Depletion of Acvr1c, a type I receptor for TGF<\/jats:styled-content>\u2010\u03b2 family of signaling molecules, led to an increase in CD<\/jats:styled-content>80 and CD<\/jats:styled-content>86 co\u2010stimulator surface expression and secreted IL<\/jats:styled-content>\u201012 in mouse bone marrow\u2010derived DC<\/jats:styled-content>s, as well as antigen\u2010specific T<\/jats:styled-content>\u2010cell proliferation.<\/jats:p>","DOI":"10.1002\/eji.201142167","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T12:46:18Z","timestamp":1336999578000},"page":"1843-1849","source":"Crossref","is-referenced-by-count":6,"title":["RNA<\/scp>i screen for kinases and phosphatases that play a role in antigen presentation by dendritic cells"],"prefix":"10.1002","volume":"42","author":[{"given":"Catarina F.","family":"Moita","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular Faculdade de Medicina Universidade de Lisboa Lisboa Portugal"}]},{"given":"\u00c2ngelo","family":"Chora","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Faculdade de Medicina Universidade de Lisboa Lisboa Portugal"}]},{"given":"Nir","family":"Hacohen","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Allergy, and Immunology Center for Immunology and Inflammatory Diseases Massachusetts General Hospital and Harvard Medical School, Boston MA USA"},{"name":"Broad institute of MIT and Harvard Cambridge MA USA"}]},{"given":"Luis F.","family":"Moita","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Faculdade de Medicina Universidade de Lisboa Lisboa Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,6,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00301.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2780"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905684106"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1157610"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.01.013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2000"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737647"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90169-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011789"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800272105"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801285105"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000059339"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.62.1.65"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.6.1693"},{"key":"e_1_2_7_16_1","unstructured":"Inaba K. Swiggard W. J. Steinman R. M. Romani N.andSchuler G. Isolation of dendritic cells.Curr. Protoc. Immunol.2001. Chapter 3: Volume 13 Unit 3.7."},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.01.040"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8463-8471.1998"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5259.263"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-633"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng154"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.201142167","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.201142167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:56:32Z","timestamp":1697504192000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.201142167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,12]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1002\/eji.201142167"],"URL":"https:\/\/doi.org\/10.1002\/eji.201142167","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,12]]}}}