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To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS\u2010CoV\u20102 detection and immunological monitoring are needed. We quantified IgM, IgG, and IgA antibodies recognizing the SARS\u2010CoV\u20102 receptor\u2010binding domain (RBD) or the Spike (S) protein over a period of 6 months following COVID\u201019 onset. We report the detailed setup to monitor the humoral immune response from over 300 COVID\u201019 hospital patients and healthcare workers, 2500 University staff, and 198 post\u2010COVID\u201019 volunteers. Anti\u2010SARS\u2010CoV\u20102 antibody responses follow a classic pattern with a rapid increase within the first three weeks after symptoms. Although titres reduce subsequently, the ability to detect anti\u2010SARS\u2010CoV\u20102 IgG antibodies remained robust with confirmed neutralization activity for up to 6 months in a large proportion of previously virus\u2010positive screened subjects. Our work provides detailed information for the assays used, facilitating further and longitudinal analysis of protective immunity to SARS\u2010CoV\u20102. Importantly, it highlights a continued level of circulating neutralising antibodies in most people with confirmed SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/eji.202048970","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T05:21:14Z","timestamp":1603257674000},"page":"2025-2040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":194,"title":["Seroprevalence of anti\u2010SARS\u2010CoV\u20102 antibodies in COVID\u201019 patients and healthy volunteers up to 6 months post disease onset"],"prefix":"10.1002","volume":"50","author":[{"given":"Patr\u00edcia","family":"Figueiredo\u2010Campos","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Birte","family":"Blankenhaus","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Catarina","family":"Mota","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"},{"name":"Centro Hospitalar Universit\u00e1rio Lisboa Norte  Lisbon Portugal"}]},{"given":"Andreia","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Marta","family":"Serrano","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Silvia","family":"Ariotti","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Catarina","family":"Costa","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Helena","family":"Nunes\u2010Caba\u00e7o","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Ant\u00f3nio M.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Pedro","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Centro Hospitalar Universit\u00e1rio Lisboa Norte  Lisbon Portugal"}]},{"given":"M. Concei\u00e7\u00e3o","family":"Pereira\u2010Santos","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Fabiana","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Jo\u00e3o Lobo Antunes Faculdade de Medicina, Universidade de Lisboa  Lisbon Portugal"}]},{"given":"Jorge","family":"Conde\u00e7o","sequence":"additional","affiliation":[{"name":"Instituto Portugu\u00eas do Sangue e Transplanta\u00e7\u00e3o (IPST)  Lisbon Portugal"}]},{"given":"M. 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