{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:21:57Z","timestamp":1761708117911},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,8,9]],"date-time":"2007-08-09T00:00:00Z","timestamp":1186617600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J Lipid Sci &amp;amp; Tech"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fatty acid pattern of ruminant edible fats is very complex and its diversity is mostly determined by extensive interactions between dietary factors and rumen metabolism. Characterization of intermediates of polyunsaturated fatty acid (PUFA) biohydrogenation in lipids from ruminants, particularly when supplemented with oils, can be a rather difficult task. In this study, we characterized some minor PUFA that occur in muscle samples from linseed oil\u2010fed lambs, by covalent adduct chemical ionization tandem mass spectrometry (CACI\u2010MS\/MS) using acetonitrile as reagent of chemical ionization. Two minor metabolites, <jats:italic>cis<\/jats:italic>\u201012,<jats:italic>cis<\/jats:italic>\u201015 18:2 and <jats:italic>cis<\/jats:italic>\u20109,<jats:italic>trans<\/jats:italic>\u201011,<jats:italic>cis<\/jats:italic>\u201015 18:3 were identified for the first time in lamb meat through CACI\u2010MS\/MS. However, the <jats:italic>cis<\/jats:italic>\u20109,<jats:italic>trans<\/jats:italic>\u201011,<jats:italic>cis<\/jats:italic>\u201015 18:3 isomer was incompletely resolved from 20:3<jats:italic>n<\/jats:italic>\u20109 under our GC\/MS conditions. Thus, in studies reporting biohydrogenation intermediates in meat, caution must be taken in order to ensure proper resolution of these two fatty acid methyl esters (FAME) and therefore avoid quantitative overestimations. CACI\u2010MS\/MS was a powerful and rapid method for the structural characterization of minor polyenoic FAME present in ruminant fat, and it seems to be a good alternative to classic 4,4\u2010dimethyloxazoline and picolinyl derivatization methods.<\/jats:p>","DOI":"10.1002\/ejlt.200700035","type":"journal-article","created":{"date-parts":[[2007,8,9]],"date-time":"2007-08-09T14:17:35Z","timestamp":1186669055000},"page":"879-883","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Identification of <b><i>cis<\/i><\/b>\u201012,<b><i>cis<\/i><\/b>\u201015 octadecadienoic acid and other minor polyenoic fatty acids in ruminant fat"],"prefix":"10.1002","volume":"109","author":[{"given":"Susana P.","family":"Alves","sequence":"first","affiliation":[]},{"given":"Rui J. B.","family":"Bessa","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2007,8,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1453-7_9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(99)00117-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1438-9312(200201)104:1<36::AID-EJLT36>3.0.CO;2-3"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac981387f"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(99)00109-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00037-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034221"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1797"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0516584"},{"key":"e_1_2_1_11_2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1438-9312(200011)102:11<667::AID-EJLT667>3.0.CO;2-N"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-997-0156-3"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-003-1499-5"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533983"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72134-8"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(05)73006-X"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20061783"}],"container-title":["European Journal of Lipid Science and Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fejlt.200700035","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ejlt.200700035","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ejlt.200700035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T08:57:35Z","timestamp":1694509055000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ejlt.200700035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1002\/ejlt.200700035"],"URL":"https:\/\/doi.org\/10.1002\/ejlt.200700035","archive":["Portico"],"relation":{},"ISSN":["1438-7697","1438-9312"],"issn-type":[{"value":"1438-7697","type":"print"},{"value":"1438-9312","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]},"assertion":[{"value":"2007-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}