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The immobilization of enzymes glucose oxidase (Gox) and horseradish peroxidase (HRP) were carried out in a very easy way. An aliquot of 10\u2005\u00b5L of a Gox\/HRP mixture was deposited on the electrode surface and left there until dried (approximately 1\u2005h) at room temperature. The ferricyanide generated enzymatically was detected amperometrically applying a potential of \u22120.1\u2005V (vs. Ag pseudo reference electrode). The sensor, so constructed, shows a good sensitivity to glucose (\u22122.12\u2005\u00b5A\/mM) with a slope deviation of \u00b10.06\u2005\u00b5A\/mM and a linear range comprised between 0.05 and 1\u2005mM of glucose, with a limit of detection of 0.025\u2005mM. These sensors show good intersensor reproducibility and a high stability. When they are stored at 4\u2009\u00b0C, no significant changes in the slope value of the glucose calibration plot were found after 3\u2005months. Glucose was determined in real samples as honey, blood, drink for babies and glucosed drink with a great accuracy.<\/jats:p>","DOI":"10.1002\/elan.201000471","type":"journal-article","created":{"date-parts":[[2010,12,7]],"date-time":"2010-12-07T11:14:24Z","timestamp":1291720464000},"page":"209-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Enzymatic Sensor Using Mediator\u2010Screen\u2010Printed Carbon Electrodes"],"prefix":"10.1002","volume":"23","author":[{"given":"Julien","family":"Biscay","sequence":"first","affiliation":[]},{"given":"Estefani\u00e1 Costa","family":"Rama","sequence":"additional","affiliation":[]},{"given":"Maria Beso\u00f1a Gonz\u00e1lez","family":"Garc\u00eda","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 Manuel Pingarr\u00f3n","family":"Carraz\u00f3n","sequence":"additional","affiliation":[]},{"given":"Austin Costa","family":"Garc\u00eda","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2010,12,7]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.07.005"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac035143t"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00236a033"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00035a016"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2005.04.009"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.03.014"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.10.010"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.09.025"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-9140(99)00275-1"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0956-5663(92)87018-K"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1081\/AL-200054036"},{"key":"e_1_2_6_12_2","first-page":"22","author":"Ruiz J. 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