{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T16:55:46Z","timestamp":1763830546975,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,3,4]],"date-time":"2013-03-04T00:00:00Z","timestamp":1362355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electroanalysis"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rituximab (RTX) is a human\/murine chimeric monoclonal antibody (mAb) that specifically targets the transmembrane protein CD20 of B\u2010cells. The oxidation mechanism of native and denatured RTX was investigated on glassy carbon electrode. The denaturing agent sodium dodecyl sulfate and the redutancts tris(2\u2010carboxyethyl)phosphine and dithiothreitol were used. Significant differences were observed for native and denatured RTX oxidation due to morphological changes and unfolding of the RTX native structure. Native RTX presented only one oxidation peak of tyrosine and tryptophan residues, whereas in denatured RTX were detected three peaks corresponding to the oxidation of tyrosine, tryptophan and histidine residues.<\/jats:p>","DOI":"10.1002\/elan.201200552","type":"journal-article","created":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T01:29:42Z","timestamp":1362446982000},"page":"1029-1034","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Direct Electrochemistry of Native and Denatured Anticancer Antibody Rituximab at a Glassy Carbon Electrode"],"prefix":"10.1002","volume":"25","author":[{"given":"Severino Carlos B.","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"In\u00eas B.","family":"Santarino","sequence":"additional","affiliation":[]},{"given":"Ana Maria","family":"Oliveira\u2010Brett","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2013,3,4]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"crossref","unstructured":"S. Nussbaumer P. Bonnabry J.\u2005L. Veuthey S. Fleury\u2010Souverain Talanta 2011 85 2265","DOI":"10.1016\/j.talanta.2011.08.034"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2006.01288.x"},{"key":"e_1_2_6_3_2","unstructured":"M.\u2005E. Reff K. Carner K.\u2005S. Chambers P.\u2005C. Chinn J.\u2005E. Leonard R. Raab R.\u2005A. Newman N. Hanna D.\u2005R. Anderson Blood199483 435."},{"key":"e_1_2_6_4_2","first-page":"12","author":"Scheinfeld N.","year":"2006","journal-title":"Dermat. Online J."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2012.06.063"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar0400443"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200200618"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0728(96)05065-6"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp992591p"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0728(80)80281-6"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.05.012"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2012.08.004"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0728(80)80448-7"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2011.02.001"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0728(80)80447-5"},{"key":"e_1_2_6_16_2","first-page":"690","volume-title":"Electrochemistry of Nucleic Acids and Proteins. Towards Electrochemical Sensors for Genomics and Proteomics","author":"Palecek E.","year":"2005"},{"volume-title":"The Basics Buffer Solutions","year":"1996","author":"Beynon R.","key":"e_1_2_6_17_2"},{"key":"e_1_2_6_18_2","doi-asserted-by":"crossref","unstructured":"J. Du H. Wang C. Zhong B. Peng M. Zhang B. Li S. Huo Y. Guo J. Ding J. Biol. Chem.2007 282 15073.","DOI":"10.1074\/jbc.M701654200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.elecom.2012.06.027"},{"volume-title":"Principles of Biochemistry","year":"2005","author":"Nelson D.","key":"e_1_2_6_20_2"},{"key":"e_1_2_6_21_2","unstructured":"W. Reiner T. Naven.Proteomics in Practice Wiley\u2010VCH Berlin2002."},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1290"}],"container-title":["Electroanalysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felan.201200552","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elan.201200552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T08:50:11Z","timestamp":1760518211000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elan.201200552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,4]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/elan.201200552"],"URL":"https:\/\/doi.org\/10.1002\/elan.201200552","archive":["Portico"],"relation":{},"ISSN":["1040-0397","1521-4109"],"issn-type":[{"type":"print","value":"1040-0397"},{"type":"electronic","value":"1521-4109"}],"subject":[],"published":{"date-parts":[[2013,3,4]]},"assertion":[{"value":"2012-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}