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The CYP1A2\u2010dsDNA interaction <jats:italic>in<\/jats:italic>\u2005<jats:italic>situ<\/jats:italic> evaluation using a DNA\u2010electrochemical biosensor and differential pulse voltammetry was investigated. A dsDNA\u2010electrochemical biosensor showed that CYP1A2 interacted with dsDNA causing conformational changes in the double helix chain and DNA oxidative damage. A preferential interaction between the dsDNA guanosine residues and CYP1A2 was found, as free guanine and 8\u2010oxoguanine, a DNA oxidative damage biomarker, oxidation peaks were detected. This was confirmed using guanine and adenine homopolynucleotides\u2010electrochemical biosensors. 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