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To contribute to this success, distinct electrochemical immunosensing platforms were developed for the analysis of the ExtraCellular Domain of the Human Epidermal growth factor Receptor 2 (HER2\u2010ECD) through sandwich assays on nanomaterial\u2010modified screen\u2010printed carbon electrodes (SPCEs). The most promising platforms showed to be SPCEs modified with (i) gold nanoparticles (AuNPs) and (ii) multiwalled carbon nanotubes combined with AuNPs. The antibody\u2010antigen interaction was detected using a secondary antibody labelled with alkaline phosphatase and 3\u2010indoxyl phosphate and silver ions as the enzymatic substrate. The electrochemical signal of the enzymatically generated metallic silver was recorded by linear sweep voltammetry. Under the optimized conditions, linear calibration plots were obtained between 7.5 and 50\u2005ng\/mL and the total assay time was 2\u2005h\u200920\u2009min, achieving LODs of 0.16\u2005ng\/mL (SPCE\u2010MWCNT\/AuNP) and 8.5\u2005ng\/mL (SPCE\u2010AuNP), which are well below the established cut\u2010off value of 15\u2005ng\/mL for this cancer biomarker.<\/jats:p>","DOI":"10.1002\/elan.201800537","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T04:24:12Z","timestamp":1543292652000},"page":"121-128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Electrochemical Sensing Platforms for HER2\u2010ECD Breast Cancer Biomarker Detection"],"prefix":"10.1002","volume":"31","author":[{"given":"Maria","family":"Freitas","sequence":"first","affiliation":[{"name":"REQUIMTE\/LAQV Instituto Superior de Engenharia do Porto Polit\u00e9cnico do Porto  Rua Dr. Ant\u00f3nio Bernardino de Almeida 431 4200-072 Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-8866","authenticated-orcid":false,"given":"Henri P. 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