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Different experimental factors were explored, such as the influence of concentration, composition and pH of the medium, mass transport, adsorption of products on GCE and the presence of possible interferers on the oxidation of 3\u2010mAde. The electrochemical data demonstrated that the methyl group is not electroactive, but strongly influences the oxidation mechanism of 3\u2010mAde. The anodic behaviour of 3\u2010mAde occurred from mass transport by diffusion, in a single irreversible pH\u2010dependent step, in an electrode reaction with the removal of one electron and one proton. By cyclic voltammetry the diffusion coefficient of 3\u2010mAde was assessed in physiological neutral pH (<jats:italic>D<\/jats:italic><jats:sub>3\u2010mAde<\/jats:sub>=1.57\u00d710<jats:sup>\u22125<\/jats:sup>\u2005cm<jats:sup>2<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>). In comparison to adenine, the oxidation of 3\u2010mAde occurred at more positive potential values (~200\u2005mV), thus allowing the simultaneous voltammetric determination of both bases. A new differential pulse voltammetric method for determination of 3\u2010mAde in acetate buffer (pH=4.5) and phosphate buffer (pH=7.0) was also proposed. Recovery experiments were performed and additions of 3\u2010mAde with known concentrations were made in samples prepared from mixtures of free DNA bases and the 7\u2010mGua adduct in acetate buffer (pH=4.5). The results were quite satisfactory (96.4 \u2013 101.3% recovery), indicating excellent precision and accuracy, as well as advantages such as simplicity, speed and cost of the proposed method.<\/jats:p>","DOI":"10.1002\/elan.202400149","type":"journal-article","created":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T22:49:20Z","timestamp":1724021360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Electrochemical Oxidation and Determination of 3\u2010Methyladenine at Glassy Carbon Electrode"],"prefix":"10.1002","volume":"37","author":[{"given":"Jos\u00e9 Eudes S.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Federal Rural University of Pernambuco Department of Chemistry  Recife-PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex P.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco Department of Chemistry  Recife-PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Gouveia S.","family":"Neto","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco Department of Chemistry  Recife-PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo B.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco Fundamental Chemistry Department  Recife, PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Souza Gil","sequence":"additional","affiliation":[{"name":"Federal University of Goi\u00e1s Faculty of Pharmacy  Goi\u00e2nia-GO Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vagner B.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco Fundamental Chemistry Department  Recife, PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Severino Carlos B.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco Department of Chemistry  Recife-PE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20200009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8AY01667D"},{"key":"e_1_2_8_4_1","first-page":"190","article-title":"\u201cUrinary Excretion of Nitrate, N-Nitrosoproline, 3-Methyladenine, and 7-Methylguanine in a Colombian Population at High Risk for Stomach Cancer\u201d","volume":"51","author":"Stillwell W. 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