{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:51:55Z","timestamp":1761580315248,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":3756,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The behavior of modified human serum albumin (HSA) from penicillin\u2010treated patients and <jats:italic>in vitro<\/jats:italic> conjugated samples was studied by isoelectric focusing in the absence and presence of 8 <jats:sc>M<\/jats:sc> urea. It is shown that samples classified as bisalbuminemic after separation under native conditions display a spectrum of several major bands with decreasing p<jats:italic>I<\/jats:italic>s when focusing is done in the presence of 8 <jats:sc>M<\/jats:sc> urea. In this spectrum, the distances between two consecutive major bands are as expected from a 2\u2010unit charge difference. Since this result is compatible with the blockage of a lysine residue with a benzylpenicilloyl (BPO) group, each major band can be interpreted as representing an HSA fraction covalently linked to different numbers of benzylpenicilloyl groups. The separation methods presented therefore seem to constitute simple and reliable tools for a detailed assessment of the extent of BPO covalent binding to HSA both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/elps.11501601174","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T10:29:35Z","timestamp":1117276175000},"page":"1031-1033","source":"Crossref","is-referenced-by-count":2,"title":["Transient bisalbuminemia: Separation by isoelectric focusing of human albumin fractions linked to different numbers of benzylpenicilloyl groups"],"prefix":"10.1002","volume":"16","author":[{"given":"Jorge","family":"Rocha","sequence":"first","affiliation":[]},{"given":"J\u00fcrgen","family":"Bohner","sequence":"additional","affiliation":[]},{"given":"Jost","family":"K\u00f6mpf","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,4,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(68)90360-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(79)90408-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.114.6.875"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.115.4.821"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.115.4.821"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)81351-1"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)81382-X"},{"key":"e_1_2_1_9_2","first-page":"363","volume":"539","author":"Yvon M.","year":"1991","journal-title":"FEBS Lett."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150120415"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00755a031"},{"key":"e_1_2_1_12_2","first-page":"196","volume-title":"Elektrophorese Forum '85","author":"Hackler R.","year":"1985"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.11501601174","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.11501601174","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T08:52:18Z","timestamp":1760518338000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.11501601174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1002\/elps.11501601174"],"URL":"https:\/\/doi.org\/10.1002\/elps.11501601174","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}