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In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and improves reproducibility and matching ratios between gels when analyzed by specialized software.<\/jats:p>","DOI":"10.1002\/elps.200500757","type":"journal-article","created":{"date-parts":[[2006,3,28]],"date-time":"2006-03-28T07:20:46Z","timestamp":1143530446000},"page":"1825-1831","source":"Crossref","is-referenced-by-count":34,"title":["Sample sonication after trichloroacetic acid precipitation increases protein recovery from cultured hippocampal neurons, and improves resolution and reproducibility in two\u2010dimensional gel electrophoresis"],"prefix":"10.1002","volume":"27","author":[{"given":"Bruno J.","family":"Manadas","sequence":"first","affiliation":[]},{"given":"Konstantinos","family":"Vougas","sequence":"additional","affiliation":[]},{"given":"Michael","family":"Fountoulakis","sequence":"additional","affiliation":[]},{"given":"Carlos B.","family":"Duarte","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,4,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(02)01996-0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10075"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200206)2:6<666::AID-PROT666>3.0.CO;2-V"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200405979"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0320511"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr025541x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.10.029"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300487"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200209)2:9<1169::AID-PROT1169>3.0.CO;2-L"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401662"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180309"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200104)1:5<699::AID-PROT699>3.0.CO;2-C"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305844"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4514"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(99)80004-4"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401178"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<660::AID-ELPS660>3.0.CO;2-Q"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<705::AID-ELPS705>3.0.CO;2-Q"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200211)2:11<1479::AID-PROT1479>3.0.CO;2-A"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000301)21:5<834::AID-ELPS834>3.0.CO;2-X"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr025564b"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401031"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.200500757","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.200500757","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:07:24Z","timestamp":1760555244000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.200500757"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,27]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1002\/elps.200500757"],"URL":"https:\/\/doi.org\/10.1002\/elps.200500757","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,27]]}}}