{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:38:34Z","timestamp":1772253514384,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T00:00:00Z","timestamp":1234137600000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A single multiplex PCR assay capable of simultaneously amplifying nine canine\u2010specific autosomal STR markers (FH3210, FH3241, FH2004, FH2658, FH4012, REN214L11, FH2010, FH2361 and the newly described C38) was developed for individual identification and parentage testing in domestic dogs. In order to increase genotyping efficiency, amplicon sizes were optimized for a 90\u2013350\u2009bp range, with fluorescently labelled primers for use in Applied Biosystems, Inc., platforms. The performance of this new multiplex system was tested in 113 individuals from a case\u2010study population and 12 random dogs from mixed\u2010breed origin. Co\u2010dominant inheritance of STR alleles was investigated in 101 father, mother and son trios. Expected heterozygosity values vary between 0.5648 for REN214L11 and 0.9050 for C38. The high level of genetic diversity observed for most markers provides this multiplex with a very high discriminating power (matching probability=1.63\/10<jats:sup>10<\/jats:sup> and matching probability among siblings=4.9\/10<jats:sup>3<\/jats:sup>). Allele sequences and a proposal for standardized nomenclature are also herein presented, aiming at implementing the use of this system in forensic DNA typing and population genetic studies. This approach resulted in an optimized and well\u2010characterized canine DNA genotyping system that is highly performing and straightforward to integrate and employ routinely. Although this STR multiplex was developed for use and tested in a case\u2010study population, the Portuguese breed C\u00e3o de Gado Transmontano, it proved to be useful for general identification purposes or parentage testing.<\/jats:p>","DOI":"10.1002\/elps.200800307","type":"journal-article","created":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T09:32:20Z","timestamp":1234171940000},"page":"417-423","source":"Crossref","is-referenced-by-count":42,"title":["A new autosomal STR nineplex for canine identification and parentage testing"],"prefix":"10.1002","volume":"30","author":[{"given":"Barbara","family":"van Asch","sequence":"first","affiliation":[]},{"given":"C\u00edntia","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Leonor","family":"Gusm\u00e3o","sequence":"additional","affiliation":[]},{"given":"V\u00e2nia","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Filipe","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio","family":"Amorim","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,2,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073906"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086432"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/nature04338","volume":"8","author":"Lindblad\u2010Toh K","year":"2005","journal-title":"Nature"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3736605"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s00414-002-0332-9","volume":"116","author":"De Munnynck K.","year":"2002","journal-title":"Int. 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