{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:24:32Z","timestamp":1761863072160,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T00:00:00Z","timestamp":1327622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Plasma amyloid\u2010\u03b2 peptide (A\u03b2) levels have been suggested as a biomarker candidate for detecting incipient AD. A\u03b2 peptides are known to be sensitive to distinct preanalytical sample handling, which calls for standardised preanalytical procedures. We investigated serum and plasma samples of 19 patients with no clinical signs of dementia for different preanalytical sample handlings. Both serum and plasma were analysed by the one\u2010dimensional A\u03b2\u2010SDS\u2010PAGE\/immunoblot, either immediately or after storage at room temperature for 24 and 48\u2009h, respectively. The panel of A\u03b21\u201337\/38\/39\/40\/42 and A\u03b22\u201340 was evaluated. In both analytical matrices, sample storage led to a significant loss of measurable peptide levels. This effect was most pronounced during the first 24\u2009h of storage and stronger in serum than in plasma. There were no significant differences between the distinct analysed A\u03b2 peptide species regarding these results. The ratios of peptides (e.g. A\u03b21\u201342\/A\u03b21\u201340 and A\u03b21\u201342\/A\u03b21\u201338) displayed a higher stability under the influence of storage than each single peptide. In conclusion, plasma may be more appropriate than serum for analysing A\u03b2 peptides for routine application. At least, the analysis should be done within 24\u2009h and peptide ratios should be created to minimise artificial results.<\/jats:p>","DOI":"10.1002\/elps.201100455","type":"journal-article","created":{"date-parts":[[2012,1,27]],"date-time":"2012-01-27T15:32:09Z","timestamp":1327678329000},"page":"445-450","source":"Crossref","is-referenced-by-count":21,"title":["Stability of amyloid\u2010\u03b2 peptides in plasma and serum"],"prefix":"10.1002","volume":"33","author":[{"given":"Mirko","family":"Bibl","sequence":"first","affiliation":[]},{"given":"Volker","family":"Welge","sequence":"additional","affiliation":[]},{"given":"Hermann","family":"Esselmann","sequence":"additional","affiliation":[]},{"given":"Jens","family":"Wiltfang","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2012,1,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00818.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200406068"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(06)70501-4"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04763.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2005.06.015"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00313.x"},{"journal-title":"Int. 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