{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T02:19:02Z","timestamp":1766888342888,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2012,7,16]],"date-time":"2012-07-16T00:00:00Z","timestamp":1342396800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Bovine theileriosis is a tick\u2010borne disease caused by one or more hemoprotozoan parasites of the genus T<\/jats:styled-content>heileria<\/jats:italic>. In the past, T<\/jats:styled-content>heileria<\/jats:italic> infection in cattle in A<\/jats:styled-content>ustralia was largely asymptomatic and recognized to be associated with T<\/jats:styled-content>heileria buffeli<\/jats:italic>. However, outbreaks of theileriosis have occurred in beef and dairy cattle in subtropical climatic regions (N<\/jats:styled-content>ew S<\/jats:styled-content>outh W<\/jats:styled-content>ales) of A<\/jats:styled-content>ustralia. There is also one published report of a recent theileriosis outbreak in a beef farm near S<\/jats:styled-content>eymour in the southeastern state of V<\/jats:styled-content>ictoria. In order to gain an improved insight into the genetic composition of T<\/jats:styled-content>heileria<\/jats:italic> populations following this outbreak, we undertook herein an integrated PCR<\/jats:styled-content>\u2010coupled mutation scanning\u2010sequencing\u2010phylogenetic analysis of sequence variation in part of the major piroplasm surface protein (MPSP<\/jats:styled-content>) gene within and among samples from cattle involved in the outbreak. Theileria<\/jats:italic> DNA<\/jats:styled-content> was detected in 89.4% of 94 cattle in the S<\/jats:styled-content>eymour farm; the genetic analysis showed that the ikeda<\/jats:italic> and chitose<\/jats:italic> genotypes representing the T<\/jats:styled-content>heileria orientalis<\/jats:italic> complex were detected in 75 and 4.8% of 84 infected cattle, respectively, and that mixed populations of these two genotypes were found in 20.2% of infected cattle. Given unpublished reports of a significant increase in the number of outbreaks in V<\/jats:styled-content>ictoria, future investigations should focus sharply on elucidating the epidemiology of T<\/jats:styled-content>heileria<\/jats:italic> to subvert the economic impact on the cattle industry in this state. Although used here to explore genetic variation within the T. orientalis<\/jats:italic> complex in A<\/jats:styled-content>ustralia, a mutation scanning\u2010based approach has broad applicability to other species of T<\/jats:styled-content>heileria<\/jats:italic> in other countries.<\/jats:p>","DOI":"10.1002\/elps.201200082","type":"journal-article","created":{"date-parts":[[2012,7,16]],"date-time":"2012-07-16T10:11:14Z","timestamp":1342433474000},"page":"2036-2040","source":"Crossref","is-referenced-by-count":42,"title":["Mutation scanning\u2010based analysis of T<\/scp>heileria orientalis<\/i> populations in cattle following an outbreak"],"prefix":"10.1002","volume":"33","author":[{"given":"Nadia","family":"Cufos","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne Parkville Victoria Australia"},{"name":"Interdisciplinary Research Centre on Animal Health, Faculty of Veterinary Medicine, Technical University of Lisbon (CIISA\/FVM\/TUL) P\u00f3lo Universit\u00e1rio do Alto da Ajuda Lisbon Portugal"}]},{"given":"Abdul","family":"Jabbar","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne Parkville Victoria Australia"}]},{"given":"Lu\u00eds M.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Centre on Animal Health, Faculty of Veterinary Medicine, Technical University of Lisbon (CIISA\/FVM\/TUL) P\u00f3lo Universit\u00e1rio do Alto da Ajuda Lisbon Portugal"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science The University of Melbourne Parkville Victoria Australia"}]}],"member":"311","published-online":{"date-parts":[[2012,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-0903-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1992.tb07450.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00113-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-22"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2009.00540.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00074-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.08.012"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.485"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.20.4354"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.8.754"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg180"},{"key":"e_1_2_6_14_1","unstructured":"D. L. Swofford 1999 Sinauer Associates Sunderland"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.9.817"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.71.937"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2011.552857"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.9.081307.164359"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1982.tb02735.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90164-F"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00008-9"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00095-2"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10-0472"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.68.1335"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.133"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb11041.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10-0263"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000067718"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(95)00074-B"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felps.201200082","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.201200082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:06:06Z","timestamp":1760522766000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.201200082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":29,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1002\/elps.201200082"],"URL":"https:\/\/doi.org\/10.1002\/elps.201200082","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"type":"print","value":"0173-0835"},{"type":"electronic","value":"1522-2683"}],"subject":[],"published":{"date-parts":[[2012,7]]}}}