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Despite the promising nature of this intrinsic echinoderm trait, it was only recently that this complex biological event started to be unveiled. In the present study, a 2<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content> gel\u2010based phosphoproteomics approach was used to investigate changes in starfish neuronal protein phosphorylation states at two different wound healing time\u2010graded events following arm tip amputation, 48 h and 13 days. Among the resolved protein spots in 3.0\u20135.6 <jats:styled-content style=\"fixed-case\">NL<\/jats:styled-content> pH <jats:styled-content style=\"fixed-case\">IEF<\/jats:styled-content> strips, 190, 142, and 124 had a phosphoprotein signal in the control and the two injury experimental groups, respectively. Gel image analysis, highlighted 129 spots with an injury\u2010related protein phosphorylation dynamics, several being exclusively phosphorylated in controls (72 spots), injured nerves (8 spots) or, showing significantly different phosphorylation ratios (37 spots). Within these, a total of 43 proteins were identified with <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>. Altogether, several intervening proteins of important injury\u2010signaling pathways that seem to be modulated through phosphorylation, were identified for the first time in starfish radial nerve cord early regeneration events. These include cytoskeleton re\u2010organization toward the formation of the neuronal growth cones; cell membrane rearrangements, actin filaments, and microtubules dynamics; mRNA binding and transport; lipid signaling; <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>otch pathway; and neuropeptide processing.<\/jats:p>","DOI":"10.1002\/elps.201200274","type":"journal-article","created":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T07:51:51Z","timestamp":1350028311000},"page":"3764-3778","source":"Crossref","is-referenced-by-count":9,"title":["Radial nerve cord protein phosphorylation dynamics during starfish arm tip wound healing events"],"prefix":"10.1002","volume":"33","author":[{"given":"Catarina F.","family":"Franco","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Universidade Nova de Lisboa  Oeiras Portugal"}]},{"given":"Renata","family":"Soares","sequence":"additional","affiliation":[{"name":"Instituto de 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