{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:59:24Z","timestamp":1760576364124,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T00:00:00Z","timestamp":1443139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["SFRH\/BPD\/66288\/2009","SFRH\/BD\/103571\/2014"],"award-info":[{"award-number":["SFRH\/BPD\/66288\/2009","SFRH\/BD\/103571\/2014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Esophageal cancers (ECs) show poor prognosis and decreased overall survival due to late diagnosis and ineffective therapeutics, urging the introduction of novel biomarkers to aid disease management. The levels of sialyl\u2010Lewis(a) antigen (sLe<jats:sup>a<\/jats:sup>) are frequently increased in digestive tumours, which has been explored in serological non\u2010invasive prognostication (CA19\u20109 test); however, with low sensitivity and specificity. Autoantibodies against cancer antigens are considered the next generation biomarkers, as they are present in circulation long before tumour\u2010associated proteins. Based on these observations we have mined the serum of EC patients (<jats:italic>n<\/jats:italic> = 7) for antibodies against sLe<jats:sup>a<\/jats:sup>\u2010glycosylated protein species. All EC were positive for sLe<jats:sup>a<\/jats:sup>, irrespectively of their histological nature but only two patients showed elevated CA19\u20109. Moreover, IgG titers, with emphasis on IgG1, were elevated in EC patients in comparison to the control group. SLe<jats:sup>a<\/jats:sup>\u2010glycoproteins were then extracted from tumours of patients with negative CA19\u20109, isolated by immunoprecipitation and blotted with patients IgG. Autoantibodies against sLe<jats:sup>a<\/jats:sup>\u2010glycosylated proteins were detected in all cases. Different SLe<jats:sup>a<\/jats:sup>\u2010glycoproteins were observed for tumours of distinct histological natures, which now require identification and validation in larger patient sets. This preliminary data suggests that antoantibodies against sLe<jats:sup>a<\/jats:sup> glycosylated proteins hold potential for non\u2010invasive diagnosis in CA19\u20109 negative cases and sets the rational for future immunoproteomic studies envisaging highly specific EC biomarkers.<\/jats:p>","DOI":"10.1002\/elps.201500270","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T13:35:10Z","timestamp":1441200910000},"page":"2902-2907","source":"Crossref","is-referenced-by-count":6,"title":["Humoral response against sialyl\u2010Le<sup>a<\/sup> glycosylated protein species in esophageal cancer: Insights for immunoproteomic studies"],"prefix":"10.1002","volume":"36","author":[{"given":"Elisabete","family":"Fernandes","sequence":"first","affiliation":[{"name":"Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology  Porto Portugal"},{"name":"Institute of Biomedical Sciences of Abel Salazar University of Porto  Porto Portugal"}]},{"given":"Andreia","family":"Peixoto","sequence":"additional","affiliation":[{"name":"Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology  Porto Portugal"}]},{"given":"Manuel","family":"Neves","sequence":"additional","affiliation":[{"name":"Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology  Porto Portugal"}]},{"given":"Lu\u00eds Pedro","family":"Afonso","sequence":"additional","affiliation":[{"name":"Department of Pathology Portuguese Institute of Oncology of Porto  Porto Portugal"}]},{"given":"L\u00facio Lara","family":"Santos","sequence":"additional","affiliation":[{"name":"Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology  Porto Portugal"},{"name":"Health School of University of Fernando Pessoa  Porto Portugal"},{"name":"Department of Surgical Oncology Portuguese Institute for Oncology  Porto Portugal"}]},{"given":"Jos\u00e9 Alexandre","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Experimental Pathology and Therapeutics Group Portuguese Institute of Oncology  Porto Portugal"},{"name":"Mass Spectrometry Centre QOPNA, Department of Chemistry of the University of Aveiro  Aveiro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2015,9,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-004-2885-3"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.2014.895668"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003329"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1989.263"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0748-7983(96)92998-4"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/epr.11.30"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28538"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2841"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25778"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27766"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01355.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-2893"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr0500814"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.10.007"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-8-81"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2878-9"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.571"},{"key":"e_1_2_5_19_1","first-page":"3659","volume":"55","author":"Ho J. 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