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In this review article, we provide the reader with the most up\u2010to\u2010date information on open source hardware and software resources enabling the construction and utilization of an \u201copen source capillary electrophoresis instrument\u201d. While CE is still underused as a separation technique, it offers unique flexibility, low\u2010cost, and high efficiency and is particularly suitable for open source instrumental development. We overview the major parts of CE instruments, such as high voltage power supplies, detectors, data acquisition systems, and CE software resources with emphasis on availability of the open source information on the web and in the scientific literature. This review is the first of its kind, revealing accessible blueprints of most parts from which a fully functional open source CE system can be built. 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