{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:24:36Z","timestamp":1777163076294,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T00:00:00Z","timestamp":1261008000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An integrated procedure for the recovery of polyhydroxybutyrate (PHB) produced by <jats:italic>Cupriavidus necator<\/jats:italic> based on the extraction with 1,2\u2010propylene carbonate was evaluated. The effect of temperature (100\u2013145\u00b0C) and contact time (15\u201345\u2009min), precipitation period, and biomass pretreatments (pH shock and\/or thermal treatments) on PHB extraction efficiency and polymer properties was evaluated. The highest yield (95%) and purity (84%) were obtained with the combination of a temperature of 130\u00b0C and a contact time of 30\u2009min, with a precipitation period of 48\u2009h. Under these conditions, PHB had a molecular weight of 7.4\u00d710<jats:sup>5<\/jats:sup>, which was the highest value obtained. Lower values (2.2\u00d710<jats:sup>5<\/jats:sup>) were obtained for higher temperatures (145\u00b0C), while lower temperatures resulted in incomplete extraction yields (45\u201354%). No further yield improvement was achieved with the pH\/heat pretreatments, but the polymer's molecular weight was increased to 1.3\u00d710<jats:sup>6<\/jats:sup>. The PHB physical properties were not significantly affected by any of the tested procedures, as shown by the narrow ranges obtained for the glass transition temperature (4.8\u20135.0\u00b0C), melting temperature (170.1\u2013180.1\u00b0C), melting enthalpy (77.8\u201388.5\u2009J\/g) and crystallinity (55\u201362%). 1,2\u2010Propylene carbonate was shown to be an efficient solvent for the extraction of PHB from biomass. The precipitation procedure was found to highly influence the polymer recovery and its molecular weight. Although polymer molecular weight and purity were improved by applying pH\/heat pretreatment to the biomass, the procedure involves the use of large amounts of chemicals, which increases the recovery costs and makes the process environmentally unfriendly.<\/jats:p>","DOI":"10.1002\/elsc.200900034","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T17:49:52Z","timestamp":1261072192000},"page":"454-461","source":"Crossref","is-referenced-by-count":134,"title":["Recovery of polyhydroxybutyrate (PHB) from <i>Cupriavidus necator<\/i> biomass by solvent extraction with 1,2\u2010propylene carbonate"],"prefix":"10.1002","volume":"9","author":[{"given":"M\u00f4nica Lady","family":"Fiorese","sequence":"first","affiliation":[]},{"given":"Filomena","family":"Freitas","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Pais","sequence":"additional","affiliation":[]},{"given":"Ana Maria","family":"Ramos","sequence":"additional","affiliation":[]},{"given":"Gl\u00e1ucia M. F.","family":"de Arag\u00e3o","sequence":"additional","affiliation":[]},{"given":"Maria A. M.","family":"Reis","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,12,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(87)90100-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(98)01194-9"},{"key":"e_1_2_6_4_2","first-page":"183","article-title":"Production of microbial polyester by fermentation of recombinant microorganisms","volume":"71","author":"Lee S. Y.","year":"2001","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260430908"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2004.01.053"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-3910(97)00176-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(98)00126-6"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2007.11.029"},{"key":"e_1_2_6_10_2","unstructured":"J. M.Baptist US Patent 3 044 942 1962."},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00152152"},{"key":"e_1_2_6_12_2","unstructured":"R. M.Lafferty E.Heinzle US Patent 4 140 741 1979."},{"key":"e_1_2_6_13_2","first-page":"14","article-title":"Extraktion eines Thermoplasten aus Bakterien","volume":"30","author":"Lafferty R. M.","year":"1977","journal-title":"Chem. Rundsch"},{"key":"e_1_2_6_14_2","unstructured":"D. P.Kanani P. J.Sherrington D. C.Steer US Patent 3 878 093 1975."},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1128\/aem.56.7.2093-2098.1990","article-title":"Production of poly(3\u2010hydroxybutyric\u2010co\u20103\u2010hydroxyvaleric) acids","volume":"56","author":"Ramsay B. A.","year":"1990","journal-title":"Appl. Environ. Microbiol"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00154625"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(97)01136-0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2008.07.002"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00500854"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1128\/aem.54.9.2325-2327.1988","article-title":"Determination of poly\u2010\u03b2\u2010hydroxybutyricpoly\u2010\u03b2\u2010hydroxyvalerate in activated sludge by gas\u2010liquid chromatography","volume":"54","author":"Comeau Y.","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_6_21_2","unstructured":"H.Satoh Biochemical mechanisms of the anaerobic substrate uptake by enhanced biological phosphorous removal activated sludges. Master Thesis University of Tokyo1991."},{"key":"e_1_2_6_22_2","unstructured":"F.Dalcanton Produ\u00e7\u00e3o extra\u00e7\u00e3o e caracteriza\u00e7\u00e3o de poli(3\u2010hidroxibutirato) porRalstonia eutrophaem diferentes substratos. Master Thesis Universidade Federal de Santa Catarina Florian\u00f3polis2006."},{"key":"e_1_2_6_23_2","volume-title":"Modern Size\u2010Exclusion Liquid Chromatography","author":"Yau W. W.","year":"1979"},{"key":"e_1_2_6_24_2","volume-title":"Polymer Handbook","author":"Kurata M.","year":"1988"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4628(19960627)60:13<2367::AID-APP8>3.0.CO;2-C"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0197-63"},{"key":"e_1_2_6_27_2","unstructured":"J. J.Liggat G. O.Brien Melt processing of BIOPOL\u00aepolymers\u2013recent advances International Symposium Bacterial Polyhydroxyalkanoates Davos Switzerland 1996 151\u2013160."},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.54.4.450-472.1990"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-3910(02)00077-0"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/macp.200300062"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2005.09.009"},{"key":"e_1_2_6_32_2","unstructured":"D.Schumann R. A.Muller US Patent 0 186 398 2003."},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051357"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie9707432"},{"key":"e_1_2_6_35_2","unstructured":"M.Katayama Y.Tabeta US Patent 4 123 426 1978."}],"container-title":["Engineering in Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felsc.200900034","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elsc.200900034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:11:29Z","timestamp":1760555489000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elsc.200900034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["10.1002\/elsc.200900034"],"URL":"https:\/\/doi.org\/10.1002\/elsc.200900034","archive":["Portico"],"relation":{},"ISSN":["1618-0240","1618-2863"],"issn-type":[{"value":"1618-0240","type":"print"},{"value":"1618-2863","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12]]}}}