{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T20:53:38Z","timestamp":1761339218067,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,6,25]],"date-time":"2012-06-25T00:00:00Z","timestamp":1340582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>Industrial\u2010scale plant cell based bioprocesses are limited because of technological challenges associated with mass transfer and mixing in the heterogeneous broth, which typically displays non\u2010<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ewtonian characteristics. Cultures of <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>entaurea calcitrapa<\/jats:italic> cells, presenting milk\u2010clotting activity, were established in a 7\u2010L stirred\u2010tank reactor (<jats:styled-content style=\"fixed-case\">STR<\/jats:styled-content>), keeping the initial mass transfer conditions constant, to gain a better understanding of the effects of the hydrodynamic environment and mass transfer on protease production. Cell suspensions grown in an <jats:styled-content style=\"fixed-case\">STR<\/jats:styled-content> equipped with one single marine propeller or two <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>ushton turbines showed the highest protease activities and biomass production, corresponding to the adequate mass transfer achieved under the tested bioreactor conditions. The key factors improving milk\u2010clotting protease production in a bioreactor were an efficient mass transfer and good bulk mixing without the formation of stagnant zones, while a compromise had to be established in relation to the hydrodynamic shear conditions.<\/jats:p>","DOI":"10.1002\/elsc.201100087","type":"journal-article","created":{"date-parts":[[2012,2,3]],"date-time":"2012-02-03T02:18:16Z","timestamp":1328235496000},"page":"441-449","source":"Crossref","is-referenced-by-count":9,"title":["Effects of the hydrodynamic environment and oxygen mass transfer on plant cell growth and milk\u2010clotting protease production in a stirred\u2010tank reactor"],"prefix":"10.1002","volume":"12","author":[{"given":"Sara","family":"Raposo","sequence":"first","affiliation":[]},{"given":"Maria Em\u00edlia","family":"Lima\u2010Costa","sequence":"additional","affiliation":[{"name":"Centre for Marine and Environmental Research (CIMA) University of Algarve  Faro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,6,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2009.03.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2000.01780.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2005.06.025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2007.11.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-005-6181-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000004396.97796.0c"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260350311"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp0702370"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-008-0233-5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-703X(00)00081-4"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2004.10.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2008.10.010"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2008.10.006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2010.01.011"},{"key":"e_1_2_8_16_1","first-page":"55","article-title":"Medium techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures","volume":"22","author":"Schenk R.","year":"1972","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"e_1_2_8_17_1","first-page":"159","volume-title":"Bioprocess Technology: Fundamentals and Applications","author":"Enfors S.\u2010O.","year":"1998"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1252\/jcej.30.718"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90553-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(91)90063-G"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004490050020"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-47865-5_5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690070103"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260080107"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012220855-3\/50007-9"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00009100"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023257108488"}],"container-title":["Engineering in Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felsc.201100087","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elsc.201100087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:38:09Z","timestamp":1760524689000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elsc.201100087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,25]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1002\/elsc.201100087"],"URL":"https:\/\/doi.org\/10.1002\/elsc.201100087","archive":["Portico"],"relation":{},"ISSN":["1618-0240","1618-2863"],"issn-type":[{"type":"print","value":"1618-0240"},{"type":"electronic","value":"1618-2863"}],"subject":[],"published":{"date-parts":[[2012,6,25]]}}}