{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T04:44:43Z","timestamp":1768279483155,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T00:00:00Z","timestamp":1409788800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Bacterial cellulose (BC) has attracted attention as a new functional material due to its excellent mechanical strength, tridimensional nanostructure, high purity, and increased water absorption, compared to plant cellulose. In this work, commercial laccase was immobilized on BC and the influence of enzyme concentration, contact time, and pH was optimized toward the recovery activity of immobilized laccase. This optimization was carried out using a 3<jats:sup>3<\/jats:sup> experimental design and response surface methodology. Enzyme concentration played a critical role in laccase immobilization. Under optimized conditions (0.15 \u03bcL L<jats:sup>\u22121<\/jats:sup> of enzyme concentration, 4.8 h of contact time, pH 5.4), the predicted and experimental response were equal to 47.88 and 49.30%, respectively. The thermal stability of the immobilized laccase was found to increase notably at 60 and 70\u00b0C presenting stabilization factor equal to 1.79 and 2.11, respectively. The immobilized laccase showed high operational stability, since it retained 86% of its initial activity after seven consecutive biocatalytic cycles of reaction with 2,2\u2032\u2010azinobis\u2010(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid). Kinetic studies showed that the values of Michaelis\u2013Menten constant and maximum reaction rate decreased upon immobilization (9.9\u2010 and 1.6\u2010fold, respectively). Globally, the use of immobilized laccase on BC offers an interesting tool for industrial biocatalytic applications.<\/jats:p>","DOI":"10.1002\/elsc.201400054","type":"journal-article","created":{"date-parts":[[2014,7,27]],"date-time":"2014-07-27T04:54:44Z","timestamp":1406436884000},"page":"500-508","source":"Crossref","is-referenced-by-count":43,"title":["Bacterial cellulose as carrier for immobilization of laccase: Optimization and characterization"],"prefix":"10.1002","volume":"14","author":[{"given":"Cl\u00e1udio J. R.","family":"Fraz\u00e3o","sequence":"first","affiliation":[{"name":"Department of Chemistry CICECO, University of Aveiro  Aveiro Portugal"}]},{"given":"Nuno H. C.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO, University of Aveiro  Aveiro Portugal"}]},{"given":"Carmen S. R.","family":"Freire","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO, University of Aveiro  Aveiro Portugal"}]},{"given":"Armando J. D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO, University of Aveiro  Aveiro Portugal"}]},{"given":"Ana M. R. B.","family":"Xavier","sequence":"additional","affiliation":[{"name":"Department of Chemistry CICECO, University of Aveiro  Aveiro Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9128-6275","authenticated-orcid":false,"given":"Ana P. 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