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A new and efficient method to prepare SF microemulsions (SF\u2010MEs) was developed by oil\u2010in\u2010water emulsions using high\u2010pressure homogenization to promote emulsification. During SF\u2010ME production, the secondary structure of SF changed to a more stable conformation (from random coil to \u03b2\u2010sheets), thus allowing the formation of small and stable (140.7 \u00b1 1.9 nm; polydispersity index, 0.25) SF microparticles (SF\u2010MPs). The efficiency of SF\u2010MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF\u2010MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF\u2010MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile.<\/jats:p>","DOI":"10.1002\/elsc.201400190","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T11:59:56Z","timestamp":1422532796000},"page":"400-409","source":"Crossref","is-referenced-by-count":2,"title":["Orange IV stabilizes silk fibroin microemulsions"],"prefix":"10.1002","volume":"15","author":[{"given":"Ana V.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]},{"given":"Vadim","family":"Volkov","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]},{"given":"Ana S.","family":"Abreu","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]},{"given":"Nuno","family":"Azoia","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]},{"given":"Cl\u00e1udia M.","family":"Botelho","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]},{"given":"Artur","family":"Cavaco\u2010Paulo","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}]}],"member":"311","published-online":{"date-parts":[[2015,3,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm3018033"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(02)00353-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms10041514"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"Jin H. 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