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BC membranes (oven dried and freeze\u2010dried) were swelled with 8% NaOH, in the absence and in the presence of ultrasound (US), for 30, 60, and 90 min. The influence of swelling conditions on both physico\u2010chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30\u00a0min at room temperature in the presence of US. Native and PEGylated laccase from <jats:italic>Myceliophthora thermophila<\/jats:italic> was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5\u20137\u00a0U\/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1\u20132\u00a0U\/gBC). 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