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To evaluate the clearing reversibility in a broad spectral range and the magnitude of the transparency created by a second treatment, the present study consisted on measuring the spectral collimated transmittance of lung tissues during a sequence of two treatments with electronic cigarette (e\u2010cig) fluid, which was intercalated with an immersion in saline. The saline immersion clearly reverted the clearing effect in the lung tissue in the spectral range between 220 and 1000\u2009nm. By a later application of a second treatment with the e\u2010cig fluid, the magnitude of the optical clearing effect was observed to be about the double as the one observed in the first treatment, showing that the molecules of the optical clearing agent might have converted some bound water into mobile water during the first treatment.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202200185-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202200185","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T04:58:36Z","timestamp":1660712316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Broadband spectral verification of optical clearing reversibility in lung tissue"],"prefix":"10.1002","volume":"16","author":[{"given":"Lu\u00eds R.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Centre of Innovation in Engineering and Industrial Technology (CIETI) School of Engineering, Polytechnic of Porto  Porto Portugal"}]},{"given":"Ricardo M.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Physics Department\u2014Polytechnic of Porto School of Engineering  Porto Portugal"}]},{"given":"Maria R.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Physics Department\u2014Polytechnic of Porto School of Engineering  Porto Portugal"}]},{"given":"Hugo F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre of Innovation in Engineering and Industrial Technology (CIETI) School of Engineering, Polytechnic of Porto  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-2694","authenticated-orcid":false,"given":"Valery V.","family":"Tuchin","sequence":"additional","affiliation":[{"name":"Science Medical Center Saratov State University  Saratov Russian Federation"},{"name":"Laboratory of Laser Molecular Imaging and Machine Learning Tomsk State University  Tomsk Russian Federation"},{"name":"Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision Mechanics and Control, FRC \u201cSaratov Research Centre of Russian Academy of Sciences\u201d  Saratov Russian Federation"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0667-3428","authenticated-orcid":false,"given":"Lu\u00eds M.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre of Innovation in Engineering and Industrial Technology (CIETI) School of Engineering, Polytechnic of Porto  Porto Portugal"},{"name":"Physics Department\u2014Polytechnic of Porto School of Engineering  Porto Portugal"}]}],"member":"311","published-online":{"date-parts":[[2022,8,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2018.01.085"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-33055-2"},{"key":"e_1_2_9_4_1","volume-title":"Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging","author":"Tuchin V. 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