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The comparison of those properties with the ones obtained for other optical clearing agents allows to perform an optimized agent selection to create optimized transparency in clinical applications. In this study, the evaluation and comparison of the diffusion properties of gadobutrol and glycerol in skeletal muscle was made, showing that although gadobutrol has a higher molar mass than glycerol, its low viscosity allows for a faster diffusion in the muscle. The characterization of the tissue dehydration and refractive index matching mechanisms of optical clearing was made in skeletal muscle, namely by the estimation of the diffusion coefficients for water, glycerol and gadobutrol. The estimated tortuosity values of glycerol (2.2) and of gadobutrol (1.7) showed a longer path\u2010length for glycerol in the muscle.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202200205-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202200205","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T05:21:50Z","timestamp":1663132910000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of optical clearing mechanisms in muscle during treatment with glycerol and gadobutrol solutions"],"prefix":"10.1002","volume":"16","author":[{"given":"Hugo F.","family":"Silva","sequence":"first","affiliation":[{"name":"Centre of Innovation in Engineering and Industrial Technology (CIETI) Polytechnic of Porto  Porto Portugal"}]},{"given":"In\u00eas S.","family":"Martins","sequence":"additional","affiliation":[{"name":"Centre of Innovation in Engineering and Industrial Technology (CIETI) Polytechnic of Porto  Porto Portugal"}]},{"suffix":"Jr","given":"Alexei A.","family":"Bogdanov","sequence":"additional","affiliation":[{"name":"A. 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