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By performing a weighted sum of the absorption spectra of proteins, DNA, oxygenated, and deoxygenated hemoglobin, lipids, water, melanin, and lipofuscin, it was possible to obtain a good match of the experimental\n                    <jats:italic>\u03bc<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    ) of both kidney conditions. The weights used in those reconstructions were estimated using the least squares method, and assuming a total water content of 77% in both kidney tissues, it was possible to calculate the concentrations of the other tissue components. It has been shown that with the development of cancer, the concentrations of proteins, DNA, oxygenated hemoglobin, lipids, and lipofuscin increase, and the concentration of melanin decreases. Future studies based on minimally invasive spectral measurements will allow cancer diagnosis using the proposed approach.\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300466-gra-0001.png\" xlink:title=\"image\"\/>\n                  <\/jats:p>","DOI":"10.1002\/jbio.202300466","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T01:11:23Z","timestamp":1707268283000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Optimized reconstruction of the absorption spectra of kidney tissues from the spectra of tissue components using the least squares method"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6042-701X","authenticated-orcid":false,"given":"Maria R.","family":"Pinheiro","sequence":"first","affiliation":[{"name":"Telecommunications and Multimedia Center (CTM) Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Department of Electrical and Computer Engineering Faculty of Engineering, Porto University  Porto Portugal"}]},{"given":"Lu\u00eds E.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Telecommunications and Multimedia Center (CTM) Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Department of Electrical and Computer Engineering Faculty of Engineering, Porto University  Porto Portugal"}]},{"given":"Isa C.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cancer Biology and Epigenetics Group Portuguese Oncology Institute of Porto  Porto Portugal"},{"name":"Department of Pathological, Cytological and Thanatological Anatomy Polytechnic of Porto \u2013 School of Health (ESS)  Porto Portugal"}]},{"given":"S\u00f3nia D.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cancer Biology and Epigenetics Group Portuguese Oncology Institute of Porto  Porto Portugal"},{"name":"Department of Pathology Santa Luzia Hospital (ULSAM)  Viana do Castelo Portugal"}]},{"given":"Rui M.","family":"Henrique","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cancer Biology and Epigenetics Group Portuguese Oncology Institute of Porto  Porto Portugal"},{"name":"Department of Pathology and Molecular Immunology Institute of Biomedical Sciences Abel Salazar, Porto University  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-2694","authenticated-orcid":false,"given":"Valery V.","family":"Tuchin","sequence":"additional","affiliation":[{"name":"Science Medical Center Saratov State University  Saratov Russia"},{"name":"A. 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