{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:07:15Z","timestamp":1768694835750,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Measuring the density of tartrazine (TZ) powder allowed to develop a protocol for fast preparation of aqueous solutions with a desired concentration. The stability time of these solutions decreases exponentially with the increase of TZ concentration: solutions with TZ concentrations below 25% remain stable for more than 24\u2009h, while the solution with 60% TZ remains stable only for 35\u2009min. To validate the developed protocol, muscle samples were immersed in the 40% TZ solution and, as expected, the tissue transparency increased smoothly and exponentially during the whole treatment of 30\u2009min. The diffusion time of TZ in ex\u00a0vivo skeletal muscle was quantitatively determined with high accuracy as <jats:italic>\u03c4<\/jats:italic>\n<jats:sub>TZ<\/jats:sub>\u2009=\u20095.39\u2009\u00b1\u20090.49\u2009min for sample thickness of 0.5\u2009mm. By measuring the refractive index of TZ solutions during preparation, it will be easier to prepare such solutions in a fast manner for future research on tissue optical clearing.<\/jats:p>","DOI":"10.1002\/jbio.202500160","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T22:48:58Z","timestamp":1751410138000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tartrazine for Optical Clearing of Tissues: Stability and Diffusion Issues"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1967-0860","authenticated-orcid":false,"given":"Ana R.","family":"Guerra","sequence":"first","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1920-6496","authenticated-orcid":false,"given":"Lu\u00eds R.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"}]},{"given":"Gon\u00e7alo O.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Physics Department Polytechnic Institute of Porto\u2014School of Engineering (ISEP)  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6042-701X","authenticated-orcid":false,"given":"Maria R.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Department of Electrical and Computer Engineering Porto University \u2013 Faculty of Engineering  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6603-7928","authenticated-orcid":false,"given":"Maria I.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Department of Electrical and Computer Engineering Porto University \u2013 Faculty of Engineering  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7479-2694","authenticated-orcid":false,"given":"Valery V.","family":"Tuchin","sequence":"additional","affiliation":[{"name":"Institute of Physics and Science Medical Center Saratov State University  Saratov Russian Federation"},{"name":"Interdisciplinary Laboratory of Biophotonics Tomsk State University  Tomsk Russian Federation"},{"name":"Institute of Precision Mechanics and Control Federal Research Center \u201cSaratov Scientific Center of the Russian Academy of Sciences\u201d  Saratov Russian Federation"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0667-3428","authenticated-orcid":false,"given":"Lu\u00eds M.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute for Systems and Computer Engineering, Technology and Science (INESC TEC)  Porto Portugal"},{"name":"Physics Department Polytechnic Institute of Porto\u2014School of Engineering (ISEP)  Porto Portugal"}]}],"member":"311","published-online":{"date-parts":[[2025,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-33055-2"},{"key":"e_1_2_9_3_1","volume-title":"Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging","author":"Tuchin V. V.","year":"2022"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2024.104671"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2023.117057"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/JOSAA.486496"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2024.115420"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102178"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1149123"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPEX.12.002966"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.479320"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20417"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.23489"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.21.8.081208"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202200205"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201800333"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSTQE.2020.3012350"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adm6869"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPTICA.546779"},{"key":"e_1_2_9_20_1","article-title":"Optical Clearing With Tartrazine Enables Deep Transscleral Imaging With Optical Coherence Tomography","volume":"29","author":"Narawane A.","year":"2024","journal-title":"Journal of Biomedical Optics"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.AP.6.6.060504"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.437098"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSTQE.2023.3255801"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.optlaseng.2008.12.005"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-85602-7.00008-X"},{"key":"e_1_2_9_26_1","volume-title":"Chemistry","author":"Chang R.","year":"2022"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.21.6.061007"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma18051035"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-018-1129-y"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10973-018-7663-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1088\/1054-660X\/23\/7\/075606"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.20.5.051019"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010025\u201001187\u2010z"},{"key":"e_1_2_9_34_1","first-page":"433","volume-title":"Meyler's Side Effects of Drugs","author":"Aronson J. K.","year":"2016"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202500160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T23:23:05Z","timestamp":1759965785000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202500160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/jbio.202500160"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202500160","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202500160"}}