{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:21:21Z","timestamp":1761675681717},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,5,29]],"date-time":"2009-05-29T00:00:00Z","timestamp":1243555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Self\u2010assembled monolayers (SAMs) prepared with mixtures of methyl\u2010 and hydroxyl\u2010terminated alkanethiols in different percentages on gold were used. The surfaces were pre\u2010immersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre\u2010immersion in plasma presented higher numbers of adherent leukocytes in the pure OH\u2010terminated SAM, whereas methyl\u2010terminated surfaces accounted for the lowest number of adherent cells. We observed a general increase in the number of adherent human leukocytes as the percentage of OH groups on the surface of the SAMs increased for all the pre\u2010immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre\u2010immersion conditions used, and this observation is particularly relevant in the case of the methyl\u2010terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre\u2010immersion in protein solutions has a determinant effect in leukocyte adhesion. \u00a9 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010<\/jats:p>","DOI":"10.1002\/jbm.a.32480","type":"journal-article","created":{"date-parts":[[2009,5,29]],"date-time":"2009-05-29T19:07:17Z","timestamp":1243624037000},"page":"12-19","source":"Crossref","is-referenced-by-count":16,"title":["Adhesion of human leukocytes on mixtures of hydroxyl\u2010 and methyl\u2010terminated self\u2010assembled monolayers: Effect of blood protein adsorption"],"prefix":"10.1002","volume":"93A","author":[{"given":"Judite N.","family":"Barbosa","sequence":"first","affiliation":[]},{"given":"M. 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