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These bodies are closely associated with the development of centrioles and of the acrosomal vesicle during spermiogenesis. After it first appears, acrosomal vesicle is linked to the nuclear envelope by electron\u2010dense material and subsequently acquires a dense core. Later the acrosomal vesicle moves to the apical pole of the cell while maintaining its connection to the nucleus. Although chromatin was highly condensed in the head of spermatozoa, one or more nuclear vacuoles within the nucleus were found to contain uncondensed chromatin fibers. Silver nitrate stains several nuclear and cytoplasmic structures. In the nucleus it stains the nucleolus, specific regions at the periphery of the chromatin, the synaptonemal complexes, the nuclear basal fossa, and the nuclear vacuoles of spermatozoa. In the cytoplasm, silver stains the cytoplasmic dense bodies, the material that connects the acrosomal vesicle to the spermatid nucleus, the spermatozoan subacrosomal and periacrosomal materials, the intercellular bridges of spermatids, and the centrioles. Silver staining is abolished by pretreatment with pronase E, suggesting that silver staining is due to protein.<\/jats:p>","DOI":"10.1002\/jmor.1051950205","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T16:08:52Z","timestamp":1109261332000},"page":"177-188","source":"Crossref","is-referenced-by-count":8,"title":["Ultrastructure and silver\u2010staining analysis of spermatogenesis in the sea urchin <i>Paracentrotus lividus<\/i> (Echinodermata, Echinoidea)"],"prefix":"10.1002","volume":"195","author":[{"given":"Mario","family":"Sousa","sequence":"first","affiliation":[]},{"given":"Carlos","family":"Azevedo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120100404"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/z74-176"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01688170.1986.10510206"},{"key":"e_1_2_1_5_1","first-page":"87","article-title":"Spermatogen\u00e8se de Nereis diversicolor O.F. 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