{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T20:00:56Z","timestamp":1769889656921,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Human papillomavirus (HPV) genotype classification relies on DNA sequence similarity to reference (prototype) sequences. Most HPV assays used for cervical cancer screening were clinically validated against European HPV prototypes. However, the impact of HPV sequence polymorphisms on test performance remains unexplored. We evaluated whether sequence variation in E6\/E7 relative to HPV prototypes affects test performance by analyzing cervicovaginal samples from 990 women enrolled (2019\u20102022) across Belgium, Portugal, Brazil and Ecuador. Samples were tested using cobas, Anyplex, and next\u2010generation sequencing (Ampliseq\/Ion Torrent targeting E6\/E7). Sequence variation was defined as the proportion of single nucleotide polymorphisms across E6\/E7 relative to reference sequence. Sequence variation was, on average, higher in HPV\u2010negative than HPV\u2010positive samples for HPV16 (0.46% vs 0.13%) and HPV18 (0.44% vs 0.37%) using cobas. Similar patterns were observed with Anyplex (HPV16: 0.78% vs 0.13%, HPV33: 0.66% vs 0.40%, HPV58: 0.79% vs 0.53, and HPV66: 1.14% vs 0.25%). For HPV45, sequence variation was, on average, higher in HPV\u2010positive than HPV\u2010negative samples when tested with Anyplex (0.87% vs 0.43%). For HPV types 18, 31, 35, 39, 51, 52, 56, 59 and 68, the mean sequence variation was similar between HPV\u2010negative and HPV\u2010positive samples using Anyplex. Our findings show that sequence variation relative to prototypes may impact test performance.<\/jats:p>","DOI":"10.1002\/jmv.70668","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:04:21Z","timestamp":1761663861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["E6\/E7 Similarity to Human Papillomavirus Prototypes and Performance of HPV Testing by Cobas 4800 HPV Test and Anyplex II HPV HR"],"prefix":"10.1002","volume":"97","author":[{"given":"Luani R.","family":"Godoy","sequence":"first","affiliation":[{"name":"Division of Cancer Epidemiology McGill University Montr\u00e9al Quebec Canada"},{"name":"Molecular Oncology Research Center Barretos Cancer Hospital Barretos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariam","family":"El\u2010Zein","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology McGill University Montr\u00e9al Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piet","family":"Cools","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizaveta","family":"Padalko","sequence":"additional","affiliation":[{"name":"Department of Clinical Biology, Microbiology and Immunology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Verberckmoes","sequence":"additional","affiliation":[{"name":"International Centre for Reproductive Health, Department of Public Health and Primary Care, Faculty of Medicine and Health Sciences Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Degomme","sequence":"additional","affiliation":[{"name":"International Centre for Reproductive 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