{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T15:12:59Z","timestamp":1695913979022},"reference-count":28,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2009,3,25]],"date-time":"2009-03-25T00:00:00Z","timestamp":1237939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methanesulfonate (MSA) is one of the products of the photo\u2010oxidation of dimethylsulfide in the atmosphere. The genes responsible for the import of MSA into the cell (<jats:italic>msm<\/jats:italic> EFGH) and for its oxidation to formaldehyde (<jats:italic>msm<\/jats:italic> ABCD) have been previously sequenced from the soil bacterium <jats:italic>Methylosulfonomonas methylovora<\/jats:italic> str. M2 while genes for an MSA monooxygenase have been sequenced from marine bacterium <jats:italic>Marinosulfonomonas methylotropha<\/jats:italic> str. TR3. We performed a sequence\u2010based screening of the Sargasso Sea metagenome for homologues of the MSA monooxygenase (MSAMO) and MSA import genes. Our search retrieved one scaffold bearing genes with high identity to the <jats:italic>msm<\/jats:italic> ABCD cluster plus two scaffolds bearing genes highly identical to the <jats:italic>msm<\/jats:italic> EFGH operon. We increased the available data by sequencing two metagenome plasmids, which revealed more <jats:italic>msm<\/jats:italic> genes. In these three cases synteny with the original <jats:italic>msm<\/jats:italic> operons was revealed. We also retrieved several singletons showing high identity to shorter segments of the <jats:italic>msm<\/jats:italic> clusters or individual <jats:italic>msm<\/jats:italic> genes. Furthermore, a characteristic 26\u2010aa internal spacer of the MsmA Rieske\u2010type motif was conserved. Our findings support the case for a significant role of MSA degraders in the marine sulfur cycle and seem to suggest that they may be prominent members of the methylotrophic community in surface ocean waters. (\u00a9 2009 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)<\/jats:p>","DOI":"10.1002\/jobm.200800223","type":"journal-article","created":{"date-parts":[[2009,3,25]],"date-time":"2009-03-25T19:10:22Z","timestamp":1238008222000},"source":"Crossref","is-referenced-by-count":5,"title":["Evidence of methanesulfonate utilizers in the Sargasso Sea metagenome"],"prefix":"10.1002","volume":"49","author":[{"given":"Elsa","family":"Leit\u00e3o","sequence":"first","affiliation":[]},{"given":"Pedro","family":"Moradas\u2010Ferreira","sequence":"additional","affiliation":[]},{"given":"Paolo","family":"De Marco","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,8,28]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3354\/meps121227"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/311455a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/330240a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1029\/93JD02732"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/350627a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.289-296.2002"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002039900124"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2004.00661.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.6.2388-2393.1995"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1128\/JB.181.7.2244-2251.1999","article-title":"Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora","volume":"181","author":"De Marco P.","year":"1999","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.1.276-283.2006"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1093857"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601301103"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8506-8513.2005"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050826"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00906.x"},{"key":"e_1_2_1_18_2","first-page":"1428","article-title":"A novel DNA modification by sulphur","volume":"57","author":"Zhou X.F.","year":"2005","journal-title":"et al."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.15.5482-5485.2005"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2005-6-6-221"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.2.599-608.2005"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.6977-6983.2004"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.06.030"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.7970404"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00798.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400538"},{"key":"e_1_2_1_27_2","first-page":"1261","article-title":"Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate\u2010demethylating bacteria from marine environments","volume":"53","author":"Gonz\u00e1lez J.M.","year":"2003","journal-title":"et al."},{"key":"e_1_2_1_28_2","first-page":"649","article-title":"Bacterial taxa that limit sulfur flux from the ocean","volume":"314","author":"Howard E.C.","year":"2006","journal-title":"et al."},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0268"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.200800223","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.200800223","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T06:21:08Z","timestamp":1695795668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.200800223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8,28]]},"references-count":28,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1002\/jobm.200800223"],"URL":"https:\/\/doi.org\/10.1002\/jobm.200800223","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8,28]]}}}