{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T12:59:57Z","timestamp":1765112397144},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Basic Microbiol."],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:label \/><jats:p>This work aims to examine the influence of the metabolic state of the yeast <jats:italic>Pichia kudriavzevii<\/jats:italic> on the susceptibility to a metals mixture (5\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> Cd, 10\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> Pb, and 5\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup>  Zn). Cells exposed to the metals mixture in the presence of 25\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup> glucose displayed a higher loss of membrane integrity and proliferation capacity, compared to cells incubated in the absence of glucose. The analysis of the effect of individual metals revealed that glucose increased the toxic effect of Cd marginally, and of Pb significantly. The increased susceptibility to heavy metals due to glucose was attenuated in the simultaneous presence of a mitochondrial respiration inhibitor such as sodium azide (NaN<jats:sub>3<\/jats:sub>). ATP\u2010depleted yeast cells, resulting from treatment with the non\u2010metabolizable glucose analogue 2\u2010deoxy\u2010<jats:sc>d<\/jats:sc>\u2010glucose, showed an increased susceptibility to heavy metals mixture. Pre\u2010incubation of yeast cells with 1 or 1.5\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup> Ca<jats:sup>2+<\/jats:sup> reduced significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) the loss of membrane integrity induced by the metals mixture. These findings contribute to the understanding of metals mechanisms of toxicity in the non\u2010conventional yeast <jats:italic>P. kudriavzevii<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jobm.201600232","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T10:43:13Z","timestamp":1465555393000},"page":"1244-1251","source":"Crossref","is-referenced-by-count":3,"title":["Influence of the metabolic state on the tolerance of <i>Pichia kudriavzevii<\/i> to heavy metals"],"prefix":"10.1002","volume":"56","author":[{"given":"Vanessa A.","family":"Mesquita","sequence":"first","affiliation":[{"name":"Bioengineering Laboratory\u2010CIETI Department of Chemical Engineering ISEP\u2014School of Engineering of Polytechnic Institute of Porto Porto 4200\u2010072 Portugal"},{"name":"Department of Biology Federal University of Lavras (UFLA) Lavras Minas Gerais Brazil"}]},{"given":"Manuela D.","family":"Machado","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory\u2010CIETI Department of Chemical Engineering ISEP\u2014School of Engineering of Polytechnic Institute of Porto Porto 4200\u2010072 Portugal"},{"name":"CEB\u2014Centre of Biological Engineering University of Minho Campus de Gualtar Braga 4710\u2010057 Portugal"}]},{"given":"Cristina F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biology Federal University of Lavras (UFLA) Lavras Minas Gerais Brazil"}]},{"given":"Eduardo V.","family":"Soares","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory\u2010CIETI Department of Chemical Engineering ISEP\u2014School of Engineering of Polytechnic Institute of Porto Porto 4200\u2010072 Portugal"},{"name":"CEB\u2014Centre of Biological Engineering University of Minho Campus de Gualtar Braga 4710\u2010057 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.2440"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-014-3188-x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-011-0671-5"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-013-0514-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-5063-y"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.03.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2454"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.38.23203"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206214200"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307413200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2008.04.022"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/41343"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1306.011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00756-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.18.11763"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-015-4326-9"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-016-0987-y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(99)00294-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4109(199911)11:17<1312::AID-ELAN1312>3.0.CO;2-I"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA15453C"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80237-3"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.7.2897-2905.1997"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-002-3758-x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-12-3335"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/w03-044"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101494108"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(81)90002-2"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1128\/aem.58.5.1592-1600.1992","article-title":"Heavy\u2010metals alter the electrokinetic properties of bacteria, yeasts, and clay\u2010minerales","volume":"58","author":"Collins Y.E.","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_7_30_1","first-page":"727","article-title":"The uptake and cellular distribution of zinc in Saccharomyces cerevisiae","volume":"133","author":"White C.","year":"1987","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803090105"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-99-2-317"},{"key":"e_1_2_7_33_1","first-page":"2533","article-title":"Protection of Saccharomyces cerevisiae against Cd2+ toxicity by Ca2+","volume":"131","author":"Kessels B.G.F.","year":"1985","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(68)90117-X"}],"container-title":["Journal of Basic Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjobm.201600232","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jobm.201600232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:49:57Z","timestamp":1694479797000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jobm.201600232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,10]]},"references-count":33,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/jobm.201600232"],"URL":"https:\/\/doi.org\/10.1002\/jobm.201600232","archive":["Portico"],"relation":{},"ISSN":["0233-111X","1521-4028"],"issn-type":[{"value":"0233-111X","type":"print"},{"value":"1521-4028","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,10]]}}}