{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:20:09Z","timestamp":1769732409764,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82030071"],"award-info":[{"award-number":["82030071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic spinal cord injury (CSCI) is a catastrophic disease of the central nervous system (CNS), resulting in partial or complete loss of neurological function. N6\u2010methyladenosine (m6A) is the most common form of reversible posttranslational modification at the RNA level. However, the role of m6A modification in CSCI remains unknown. In this study, we established a CSCI model using a water\u2010absorbable polyurethane polymer, with behavioral assessment, electrophysiological analysis, and histochemical staining for validation. Methylated RNA immunoprecipitation sequencing (meRIP\u2010seq) and messenger RNA sequencing (mRNA\u2010seq) were jointly explored to compare the differences between CSCI spinal tissue and normal spinal tissue. Furthermore, real\u2010time quantitative reverse transcription pcr (qRT\u2013PCR), western blot analysis, and immunofluorescence staining were used to analyze m6A modification\u2010related proteins. We found that water\u2010absorbable polyurethane polymer simulated well chronic spinal cord compression. Basso mouse scale scores and electrophysiological analysis showed continuous neurological function decline after chronic compression of the spinal cord. meRIP\u2010seq identified 642 differentially modified m6A genes, among which 263 genes were downregulated and 379 genes were upregulated. mRNA\u2010seq showed that 1544 genes were upregulated and 290 genes were downregulated after CSCI. Gene Ontology\u00a0terms and enriched Kyoto Encyclopedia of Genes and Genomes\u00a0pathways were also identified. qRT\u2013PCR, western blotting, and immunofluorescence staining showed that Mettl14, Ythdf1, and Ythdf3 were significantly upregulated after CSCI. Our study revealed a comprehensive profile of m6A modifications in CSCI which may act as a valuable key for future research on CSCI.<\/jats:p>","DOI":"10.1002\/jor.25457","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T09:20:09Z","timestamp":1665134409000},"page":"1320-1334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive analysis of m6A methylation modification in chronic spinal cord injury in mice"],"prefix":"10.1002","volume":"41","author":[{"given":"Chengjun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Spine Surgery and Orthopaedics, Xiangya Hospital Central South University  Changsha China"},{"name":"Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan Province  Changsha China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University  Changsha 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