{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T12:45:12Z","timestamp":1774010712254,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Selenium (Se) deficiency is a widespread nutritional problem. Agronomic biofortification of microgreens through seed nutripriming is a promising approach to enhance Se intake. Microgreens, comprising nutrient\u2010dense foods that can be produced year\u2010round, are ideal targets for biofortification and may help alleviate malnutrition. This study evaluated the effects of Se nutripriming on germination, growth performance, chemical composition and antioxidant properties of pea (\n Pisum sativum<\/jats:italic>\n L.) microgreens.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Nutripriming significantly increased seed Se content, with the 100\u2009\u03bc\n m<\/jats:sc>\n \u201312\u2010h Se treatment resulting in a four\u2010fold accumulation compared to controls, at the same time as preserving membrane integrity. Microgreens from primed seeds exhibited enhanced Se translocation to aerial tissues and an improved mineral profile. Short\u2010term treatments (6\u2009h) increased glucose and oxalic acid levels, whereas prolonged priming (12\u2009h) favored sucrose accumulation. However, this 12\u2010h duration significantly reduced the emergence rate and aerial biomass yield. The optimal antioxidant profile, characterized by the highest total phenolic content and strongest inhibition of lipid peroxidation, was achieved with the 50\u2009\u03bc\n m<\/jats:sc>\n \u201312\u2010h Se treatment.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Se nutripriming enhanced the nutritional quality of pea microgreens but revealed a critical trade\u2010off: although 12\u2010h priming maximized Se uptake and antioxidant properties, it severely reduced agronomic production. Conversely, 6\u2010h priming promoted better biomass yield, although germination remained lower than in the NP control. Therefore, optimizing priming duration is essential to balance the biofortification benefits with overall crop productivity. \u00a9 2026 The Author(s).\n Journal of the Science of Food and Agriculture<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.70595","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T10:40:41Z","timestamp":1774003241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selenium biofortification of pea (\n \n Pisum sativum<\/i>\n <\/scp>\n L.) microgreens through seed priming: Effects on agronomic performance and nutritional quality"],"prefix":"10.1002","author":[{"given":"C\u00e1tia","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"CIMO, LA SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia Bragan\u00e7a Portugal"},{"name":"Escola Superior Agr\u00e1ria (ESA), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia Bragan\u00e7a Portugal"}]},{"given":"Matilde","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CIMO, LA SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia Bragan\u00e7a Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7523-1637","authenticated-orcid":false,"given":"Jos\u00e9","family":"Pinela","sequence":"additional","affiliation":[{"name":"CIMO, LA SusTEC, Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia Bragan\u00e7a Portugal"},{"name":"National Institute for Agricultural and Veterinary Research (INIAV, I.P.), Rua dos L\u00e1gidos, Lugar da Madalena Vila do Conde Portugal"}]}],"member":"311","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s43994-024-00177-3","article-title":"Securing a sustainable future: the climate change threat to agriculture, food security, and sustainable development goals","volume":"11","author":"Saleem A","year":"2025","journal-title":"J Umm Al\u2010Qura Univ Appl Sci"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.agsy.2023.103634","article-title":"Challenges and potential pathways towards sustainable agriculture within the European green Deal","volume":"207","author":"Boix\u2010Fayos C","year":"2023","journal-title":"Agric Syst"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.2174\/157340110793663725","article-title":"Selenium deficiency in soils and crops and its impact on animal and human health","volume":"6","author":"Gupta UC","year":"2010","journal-title":"Curr 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