{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:11:04Z","timestamp":1790989864246,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,2,10]],"date-time":"2016-02-10T00:00:00Z","timestamp":1455062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Several human studies have demonstrated occurrence of two major collagen peptides, prolyl\u2010hydroxyproline (Pro\u2010Hyp) and hydroxyprolyl\u2010glycine (Hyp\u2010Gly), in human peripheral blood. Some\n                      <jats:italic>in vitro<\/jats:italic>\n                      studies have demonstrated that Pro\u2010Hyp and Hyp\u2010Gly exert chemotaxis on dermal fibroblasts and enhance cell proliferation. Additionally, Pro\u2010Hyp enhances the production of hyaluronic acid by dermal fibroblasts. These findings suggest that the amounts of Pro\u2010Hyp and Hyp\u2010Gly in blood are important factors to show the efficacy of collagen hydrolysates on skin health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We conducted a randomised double\u2010blind placebo\u2010controlled clinical trial of ingestion of two types of collagen hydrolysates, which are composed of different amounts of the bioactive dipeptides Pro\u2010Hyp and Hyp\u2010Gly, to investigate their effects on the improvement of skin conditions. Improvement in skin conditions, such as skin moisture, elasticity, wrinkles, and roughness, were compared with a placebo group at baseline, and 4 and 8 weeks after the start of the trial. In addition, the safety of dietary supplementation with these peptides was evaluated by blood test. Collagen hydrolysate with a higher content of bioactive collagen peptides (H\u2010\n                      <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>\n                      ) showed significant and more improvement than the collagen hydrolysate with a lower content of bioactive collagen peptides (L\u2010\n                      <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>\n                      ) and the placebo, in facial skin moisture, elasticity (\n                      <jats:styled-content style=\"fixed-case\">R2<\/jats:styled-content>\n                      ), wrinkles and roughness, compared with the placebo group. In addition, there were no adverse events during the trial.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>This study demonstrated that the use of the collagen hydrolysate with a higher content of Pro\u2010Hyp and Hyp\u2010Gly led to more improvement in facial skin conditions, including facial skin moisture, elasticity, wrinkles and roughness. \u00a9 2016 Society of Chemical Industry<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.7606","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T11:03:11Z","timestamp":1454497391000},"page":"4077-4081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double\u2010blind placebo\u2010controlled clinical study"],"prefix":"10.1002","volume":"96","author":[{"given":"Naoki","family":"Inoue","sequence":"first","affiliation":[{"name":"Peptide Division Nitta Gelatin Inc.  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihito","family":"Sugihara","sequence":"additional","affiliation":[{"name":"Peptide Division Nitta Gelatin Inc.  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Skin Disease Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050206p"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf062834s"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/09637480903257711"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.05.066"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2011.09.024"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.35.422"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104694"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.90050"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.2.871"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf802785h"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2010.00827.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70112-4"},{"key":"e_1_2_6_14_1","first-page":"67","article-title":"Clinical effects of ingesting collagen hydrolysate on facial skin properties","volume":"43","author":"Sugihara F","year":"2015","journal-title":"Jpn Pharmacol Ther"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.12.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610-0387.2008.06639.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.52.211"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2010.01782.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2010.0085"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb04975.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000355523"},{"key":"e_1_2_6_22_1","article-title":"Efficacy of collagen peptide ingestion on skin. A bible for measurement and evaluation on skin","author":"Inoue N","year":"2013","journal-title":"Technical Information Institute"},{"key":"e_1_2_6_23_1","first-page":"153","article-title":"Clinical effects of collagen hydrolysates ingestion on UV\u2010induced pigmented spots of human skin: A preliminary study","volume":"28","author":"Sugihara F","year":"2012","journal-title":"Health Sci"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.01052.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.02.004"},{"key":"e_1_2_6_26_1","first-page":"227","article-title":"Effects of food-derived collagen peptides on the expression of keratin and keratin\u2010associated protein genes in the Mouse Skin","volume":"28","author":"Le Vu P","year":"2015","journal-title":"J Pharm Pharmacol"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjsfa.7606","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.7606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:57:50Z","timestamp":1760875070000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.7606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,10]]},"references-count":25,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1002\/jsfa.7606"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.7606","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,10]]},"assertion":[{"value":"2015-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}