{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:41:58Z","timestamp":1760920918399,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2010,6,23]],"date-time":"2010-06-23T00:00:00Z","timestamp":1277251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An HPLC\u2010PAD\u2010atmospheric pressure chemical ionization\u2010MS metabolite profiling analysis was conducted on the marine echinoderm <jats:italic>Marthasterias glacialis<\/jats:italic> (spiny sea\u2010star). Bio\u2010guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV\u2013Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL\u20102H3 (IC<jats:sub>25<\/jats:sub>=268\u2009\u03bcg\/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC<jats:sub>25<\/jats:sub>=411\u2009\u03bcg\/mL)) cell line, however, toxicity was lower, as it is desired for anti\u2010cancer molecules. This study suggests that <jats:italic>M. glacialis<\/jats:italic> may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.<\/jats:p>","DOI":"10.1002\/jssc.201000197","type":"journal-article","created":{"date-parts":[[2010,6,23]],"date-time":"2010-06-23T07:42:35Z","timestamp":1277278955000},"page":"2250-2257","source":"Crossref","is-referenced-by-count":13,"title":["HPLC\u2010PAD\u2010atmospheric pressure chemical ionization\u2010MS metabolite profiling of cytotoxic carotenoids from the echinoderm <i>Marthasterias glacialis<\/i> (spiny sea\u2010star)"],"prefix":"10.1002","volume":"33","author":[{"given":"Federico","family":"Ferreres","sequence":"first","affiliation":[]},{"given":"David M.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Angel","family":"Gil\u2010Izquierdo","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Valent\u00e3o","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Botelho","sequence":"additional","affiliation":[]},{"given":"Teresa","family":"Mouga","sequence":"additional","affiliation":[]},{"given":"Paula B.","family":"Andrade","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2010,7,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac199163010001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200800053"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2004.12.006"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0025315403007513h"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac198254101935"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00034992"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/157341109787047862"},{"key":"e_1_2_6_9_2","first-page":"38","volume-title":"Chemistry and Biochemistry of Plant Pigments","author":"Davies B. 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