{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:00:49Z","timestamp":1770220849911,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,24]],"date-time":"2012-04-24T00:00:00Z","timestamp":1335225600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"L.J.G. Silva","award":["SFRH\/BPD\/62877\/2009"],"award-info":[{"award-number":["SFRH\/BPD\/62877\/2009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,4]]},"abstract":"Due to strong implications for food safety, control of fluoroquinolones residues in swine meat should be undertaken to verify compliance of the contamination levels with the maximum residue limits recently updated by C<\/jats:styled-content>ommission R<\/jats:styled-content>egulation (EU<\/jats:styled-content>) N<\/jats:styled-content>o. 37\/2010 of 22 D<\/jats:styled-content>ecember 2009. Solid\u2010phase extraction is widely used in antibiotic analysis in food of animal origin. In this study, the results of a comparative study using different types of solid\u2010phase extraction columns, HLB<\/jats:styled-content>, MCX<\/jats:styled-content>, and MAX<\/jats:styled-content>, for ciprofloxacin and enrofloxacin analysis, in pork meat, are presented. In addition, diverse sample treatments for defatting, precipitate proteins, eliminate cations, and increase the ionic strength, were used to obtain the most suitable method of analysis. Only the MCX<\/jats:styled-content>'s use followed by liquid chromatography with fluorescence detection resulted in chromatograms that allow the quantification at maximum residue limits. The validation method, in terms of CC<\/jats:styled-content>\u03b1 and CC<\/jats:styled-content>\u03b2, recovery and precision determination, was according to the EU D<\/jats:styled-content>ecision 2002\/657\/EC<\/jats:styled-content>. This procedure was used in the analysis of 50 samples of pork meat of P<\/jats:styled-content>ortuguese origin. Only two samples presented residues of enrofloxacin at 30 and 42 \u03bcg\/kg, values under the legal maximum residue limit.<\/jats:p>","DOI":"10.1002\/jssc.201100971","type":"journal-article","created":{"date-parts":[[2012,4,24]],"date-time":"2012-04-24T14:32:16Z","timestamp":1335277936000},"page":"832-838","source":"Crossref","is-referenced-by-count":17,"title":["Comparison of three solid\u2010phase extraction processes in quantification of ciprofloxacin and enrofloxacin in pork meat"],"prefix":"10.1002","volume":"35","author":[{"given":"Jes\u00fas","family":"Blesa","sequence":"first","affiliation":[{"name":"Food Laboratory, Faculty of Pharmacy University of Valencia Burjassot Valencia Spain"}]},{"given":"Liliana J.G.","family":"Silva","sequence":"additional","affiliation":[{"name":"Group of Health Surveillance, CEF, Faculty of Pharmacy, University of Coimbra Coimbra Portugal"}]},{"given":"Celeste M.","family":"Lino","sequence":"additional","affiliation":[{"name":"Group of Health Surveillance, CEF, Faculty of Pharmacy, University of Coimbra Coimbra Portugal"}]},{"given":"Guillermina","family":"Font","sequence":"additional","affiliation":[{"name":"Food Laboratory, Faculty of Pharmacy University of Valencia Burjassot Valencia Spain"}]},{"given":"Angelina","family":"Pena","sequence":"additional","affiliation":[{"name":"Group of Health Surveillance, CEF, Faculty of Pharmacy, University of Coimbra Coimbra Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,4,24]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"World Health Organization (WHO) Report No. WHO\/EMC\/ZDI\/98 WHO Geneva1998."},{"key":"e_1_2_6_3_1","unstructured":"Food and Drug Administration (FDA) FDA Announces Final Decision about Veterinary Medicine FDA Silver Spring MD2005."},{"key":"e_1_2_6_4_1","first-page":"1","article-title":"Regulation No. 37\/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin","volume":"15","author":"EU Commission","year":"2010","journal-title":"Off. J. Eur. Union"},{"key":"e_1_2_6_5_1","unstructured":"European Medicines Agency (EMA) EMA\/CVMP\/SAGAM\/184651\/2005 EMA London2007."},{"key":"e_1_2_6_6_1","first-page":"10","article-title":"Directive 96\/23\/EC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products and repealing Directives 85\/358\/EEC and 86\/469\/EEC and Decisions 89\/187\/EEC and 91\/664\/EEC","volume":"125","author":"EU Council","year":"1996","journal-title":"Off. J. Eur. Union"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200500219"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-007-0208-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2004.02.065"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.01.029"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2020-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/B915676C"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.10.009"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.06.014"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.10.063"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3819-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.09.031"},{"issue":"1","key":"e_1_2_6_18_1","first-page":"1","volume":"2","author":"Leyva S.","year":"2008","journal-title":"Bol. Soc. Quim. M\u00e9x."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00212-5"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.05.007"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac061006v"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.06.026"},{"key":"e_1_2_6_23_1","first-page":"8","article-title":"Decision 2002\/657\/EC of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of results","volume":"221","author":"EU Commission","year":"2002","journal-title":"Off. J. Eur. Union"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201100971","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjssc.201100971","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.201100971","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:21:18Z","timestamp":1760876478000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.201100971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/jssc.201100971"],"URL":"https:\/\/doi.org\/10.1002\/jssc.201100971","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}