{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:35:10Z","timestamp":1772753710653,"version":"3.50.1"},"reference-count":70,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T00:00:00Z","timestamp":1342483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>Hop (<jats:italic>Humulus lupulus<\/jats:italic> L., <jats:italic>Cannabaceae<\/jats:italic> family) is prized for its essential oil contents, used in beer production and, more recently, in biological and pharmacological applications. In this work, a method involving headspace solid\u2010phase microextraction and gas chromatography\u2013mass spectrometry was developed and optimized to establish the terpenoid (monoterpenes and sesquiterpenes) metabolomic pattern of hop\u2010essential oil derived from <jats:italic>Saaz<\/jats:italic> variety as a mean to explore this matrix as a powerful biological source for newer, more selective, biodegradable and naturally produced antimicrobial and antioxidant compounds.<\/jats:p><jats:p>Different parameters affecting terpenoid metabolites extraction by headspace solid\u2010phase microextraction were considered and optimized: type of fiber coatings, extraction temperature, extraction time, ionic strength, and sample agitation. In the optimized method, analytes were extracted for 30 min at 40\u00b0C in the sample headspace with a 50\/30 \u03bcm divinylbenzene\/carboxen\/polydimethylsiloxane coating fiber. The methodology allowed the identification of a total of 27 terpenoid metabolites, representing 92.5% of the total <jats:italic>Saaz<\/jats:italic> hop\u2010essential oil volatile terpenoid composition. The headspace composition was dominated by monoterpenes (56.1%, 13 compounds), sesquiterpenes (34.9%, 10), oxygenated monoterpenes (1.41%, 3), and hemiterpenes (0.04%, 1) some of which can probably contribute to the hop of <jats:italic>Saaz<\/jats:italic> variety aroma. Mass spectrometry analysis revealed that the main metabolites are the monoterpene \u03b2\u2010myrcene (53.0 \u00b1 1.1% of the total volatile fraction), and the cyclic sesquiterpenes, \u03b1\u2010humulene (16.6 \u00b1 0.8%), and \u03b2\u2010caryophyllene (14.7 \u00b1 0.4%), which together represent about 80% of the total volatile fraction from the hop\u2010essential oil. These findings suggest that this matrix can be explored as a powerful biosource of terpenoid metabolites.<\/jats:p>","DOI":"10.1002\/jssc.201200244","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T03:01:48Z","timestamp":1342666908000},"page":"2282-2296","source":"Crossref","is-referenced-by-count":49,"title":["Headspace solid\u2010phase microextraction combined with mass spectrometry as a powerful analytical tool for profiling the terpenoid metabolomic pattern of hop\u2010essential oil derived from Saaz variety"],"prefix":"10.1002","volume":"35","author":[{"given":"Jo\u00e3o","family":"Gon\u00e7alves","sequence":"first","affiliation":[]},{"given":"Jos\u00e9","family":"Figueira","sequence":"additional","affiliation":[]},{"given":"F\u00e1tima","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 S.","family":"C\u00e2mara","sequence":"additional","affiliation":[{"name":"CQM\/UMa\u2014Centro de Qu\u00edmica da Madeira Centro de Ci\u00eancias Exactas e da Engenharia, Universidade da Madeira Campus Universit\u00e1rio da Penteada  Funchal Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,7,17]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1222","volume":"3","author":"Karuppusamy S.","year":"2009","journal-title":"J. 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