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The preparation and purification of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> is a critical step for further application, since the quality of the template is crucial to ensure reproducibility and biological relevance. Therefore, the establishment of new tools that allows the isolation of pure RNA with high quality is of particular importance. New chromatographic strategies for <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> purification were considered, exploiting affinity interactions between amino acids and nucleic acids. In the present study, a single arginine\u2010affinity chromatography step was employed for the purification of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from a total eukaryotic nucleic acid extract, thus eliminating several steps compared with current <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolation procedures. The application of this process resulted in a high <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> recovery yield of 96 \u00b1 17% and the quality control analysis revealed a high integrity (28S:18S ratio = 1.96) in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> preparations as well as a good purity, demonstrated by the scarce detection of proteins and the reduction on genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> contamination to residual concentrations. Furthermore, the performance of the new <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolation method was tested regarding the applicability of the isolated <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in modern molecular biology techniques. Hence, this new affinity approach will simplify the isolation and purification of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, which can bring great improvements in biomedical investigation.<\/jats:p>","DOI":"10.1002\/jssc.201200338","type":"journal-article","created":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T04:51:25Z","timestamp":1348462285000},"page":"3217-3226","source":"Crossref","is-referenced-by-count":17,"title":["A new strategy for <scp>RNA<\/scp> isolation from eukaryotic cells using arginine affinity chromatography"],"prefix":"10.1002","volume":"35","author":[{"given":"Rita","family":"Martins","sequence":"first","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior  Covilh\u00e3 Portugal"}]},{"given":"Cl\u00e1udio J.","family":"Maia","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior  Covilh\u00e3 Portugal"}]},{"given":"Jo\u00e3o A.","family":"Queiroz","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior  Covilh\u00e3 Portugal"}]},{"given":"Fani","family":"Sousa","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior  Covilh\u00e3 Portugal"}]}],"member":"311","published-online":{"date-parts":[[2012,9,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/80729"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/574398"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.14712\/fb2009055060243","volume":"55","author":"Vomelova I.","year":"2009","journal-title":"Folia Biol."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.2.e7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2278606"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.342607"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2009.03.042"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1862210"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.528007"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj084"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2008.05.005"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201000347"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.08.021"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20060082"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1179"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.11.002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1272"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800690"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.1078"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1729"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1097"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.12.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21383"},{"key":"e_1_2_7_28_1","volume-title":"RNA Methodologies","author":"Farrell R. 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