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Nanotechnological approaches enhance the delivery of these drugs. Therefore, a high\u2010performance liquid chromatography method was developed for the simultaneous determination of dapsone and clofazimine loaded in nanoformulations for quality control purposes. Chromatographic separation was achieved on a reversed\u2010phase Kinetex core\u2010shell C18 column, followed by spectrophotometric detection at 280\u00a0nm. Considering the different physicochemical properties of dapsone and clofazimine, elution was performed in gradient mode using an aqueous acetate buffer (50\u00a0mmol\/L, pH 4.8) and an increasing acetonitrile content from 27 to 63% v\/v at a flow rate of 1.0\u00a0mL\/min with retention times of 6.2 and 14.0\u00a0min, respectively. The method was validated according to the European Medicines Agency guideline and it was found to be specific, accurate (99.6\u2013114.0%), and precise for intra\u2010 (RSD\u00a0\u2264\u00a01.8%) and interday assays (RSD\u00a0\u2264\u00a012.5%). Both drugs showed stability after 24\u00a0h at room temperature and over three freeze\u2013thaw cycles with recoveries \u226586.2%. Low temperature (4\u00b0C) in the autosampler caused the precipitation of clofazimine and must be avoided. The validated method was successfully applied in the quantification of both drugs in nanoformulations.<\/jats:p>","DOI":"10.1002\/jssc.201800427","type":"journal-article","created":{"date-parts":[[2018,7,14]],"date-time":"2018-07-14T00:42:54Z","timestamp":1531528974000},"page":"3382-3388","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chromatographic method for the simultaneous quantification of dapsone and clofazimine in nanoformulations"],"prefix":"10.1002","volume":"41","author":[{"given":"Sandia","family":"Machado","sequence":"first","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia Universidade do Porto  Porto Portugal"}]},{"given":"Sara R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia Universidade do Porto  Porto Portugal"},{"name":"N\u00facleo de Investiga\u00e7\u00e3o e Interven\u00e7\u00e3o em Farm\u00e1cia (NIIF), Centro de Investiga\u00e7\u00e3o em Sa\u00fade e Ambiente (CISA), Escola Superior de Sa\u00fade Instituto Polit\u00e9cnico do Porto  Porto Portugal"}]},{"given":"Luise L.","family":"Chaves","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia Universidade do Porto  Porto Portugal"}]},{"given":"Sofia A. C.","family":"Lima","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia Universidade do Porto  Porto Portugal"}]},{"given":"Eduarda M. 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