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A deeper understanding of the influence of the surface topology on a molecular\/nanoscale level on adsorption is essential for further improvement. It is not clear if the binding is homogenous throughout the entire bead network. We followed the protein absorption process and observed the formation of a protein layer on fibers of chromatography resin in a time\u2010resolved manner in nanoscale. To characterize the changes in the antibody\u2010protein\u2010A ligand complex, small angle X\u2010ray scattering was employed using a miniaturized X\u2010ray\u2010transparent chromatography column packed with a MabSelect SuRe resin. Antibody\u2010free MabSelect SuRe resin fiber had an average radius of 12\u00a0nm and the protein layer thickness resulting from antibody adsorption was 5.5 and 10.4\u00a0nm for fiber and junctions, respectively under applied native conditions. 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