{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T13:58:19Z","timestamp":1769003899051,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T00:00:00Z","timestamp":1550188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Estrone, 17\u03b2\u2010estradiol and 17\u03b1\u2010ethinylestradiol are increasingly recognised as important micropollutants to be monitored in wastewater treatment plants. These estrogens are retained onto sludge due to their high adsorption and since they are largely used in land applications, the monitoring of these chemicals in sludge samples is of great importance. This study describes a method for the determination of estrone and 17\u03b1\u2010ethinylestradiol in fresh sludge samples. After spiking fresh digested sludge with estrone and 17\u03b1\u2010ethinylestradiol and maintaining in contact during 5, 30 and 60\u00a0min, the freeze\u2010dried samples were subjected to ultrasonic liquid extraction, with methanol and acetone, and analysed by high\u2010performance liquid chromatography with fluorescence detection. The average recoveries obtained for estrone and 17\u03b1\u2010ethinylestradiol using the different contact times were 103\u00a0\u00b1\u00a03 and 97\u00a0\u00b1\u00a04%, respectively. Fresh sludge samples from one waste water treatment plant located in Portugal were analysed and estrone was detected in primary fresh sludge, anaerobic digested sludge and dehydrated sludge at a concentration in the range of 1\u20134.8\u00a0\u03bcg\/g. The method here developed does not require any sample clean\u2010up, being fast and simple, reliable and inexpensive, making possible its application for monitoring the contamination of sludge with these estrogens.<\/jats:p>","DOI":"10.1002\/jssc.201801114","type":"journal-article","created":{"date-parts":[[2019,1,31]],"date-time":"2019-01-31T05:43:52Z","timestamp":1548913432000},"page":"1585-1592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Determination of estrone and 17\u03b1\u2010ethinylestradiol in digested sludge by ultrasonic liquid extraction and high\u2010performance liquid chromatography with fluorescence detection"],"prefix":"10.1002","volume":"42","author":[{"given":"Vit\u00f3ria L.","family":"Louros","sequence":"first","affiliation":[{"name":"CESAM &amp; Department of Environment and Planning University of Aveiro  Aveiro 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