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Research on analytical methods that explore urocanic acid isomerization is indispensable to fully understand the deleterious effects mediated by this biomarker. In this context, the current relevant analytical methods for determination of these isomers in human samples are summarized in this review. The methods presented here are applicable to human samples collected by noninvasive methods (or minimally invasive), encompassing an array of analytical techniques, including high\u2010performance capillary electrophoresis, confocal Raman spectroscopy, gas chromatography, high\u2010performance liquid chromatography, and mass spectrometry, among others. Developed high\u2010performance liquid chromatography methods have proven to be advantageous, allowing noninvasive collections for in vivo analysis and the confocal Raman, specially, for real\u2010time analysis. Among all these methods, high\u2010performance liquid chromatography is the most investigated one with mass spectrometry or ultraviolet detector, and the mass spectrometry detector being the most studied in the last years, demonstrating high sensitivity, very low detection limits, and accurate identification, especially for clinical investigations.<\/jats:p>","DOI":"10.1002\/jssc.202000713","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T07:22:39Z","timestamp":1602660159000},"page":"438-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Analytical tools for urocanic acid determination in human samples: A review"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3563-4105","authenticated-orcid":false,"given":"Fabiana Vieira","family":"Lima","sequence":"first","affiliation":[{"name":"Division to Faculty of Pharmacy Department of Healthy Science University of Esp\u00edrito Santo  S\u00e3o Mateus Brazil"},{"name":"Department of Pharmacy, Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7111-1029","authenticated-orcid":false,"given":"T\u00e9rcio Elyan Azevedo","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Health Sciences Institute Paulista University  S\u00e3o Paulo Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3263-1731","authenticated-orcid":false,"given":"Ana Lucia","family":"Morocho\u2010J\u00e1come","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1498-048X","authenticated-orcid":false,"given":"Isabel Filipa","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Faculty of Pharmacy UCIBIO\/REQUIMTE, MedTech\u2010Laboratory of Pharmaceutical Technology, University of Porto  Porto Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-6213","authenticated-orcid":false,"given":"Catarina Fialho","family":"Rosado","sequence":"additional","affiliation":[{"name":"CBIOS Universidade Lus\u00f3fona's Research Center for Biosciences and Health Technologies  Lisbon Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4950-8510","authenticated-orcid":false,"given":"Maria Val\u00e9ria Robles","family":"Velasco","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmaceutical Sciences University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9197-3024","authenticated-orcid":false,"given":"Andr\u00e9 Rolim","family":"Baby","sequence":"additional","affiliation":[{"name":"Department 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