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Maximum strain at break and tensile strength of the developed fibers were found to be 8.5% and 204.9 MPa, respectively. After 14 d of immersion in simulated body fluid (SBF), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and inductively coupled plasma emission (ICP) spectroscopy analyses showed that a bioactive Ca\u2010P layer was formed on the surface of the fibers, meaning that they exhibit a bioactive behavior. The samples showed around 120% max. swelling in physiological conditions. The pore sizes of 3\u2010D chitosan fiber mesh scaffolds were observed to be in the range of 100\u2013500 \u03bcm by SEM. The equilibrium\u2010swelling ratio of the developed scaffolds was found to be around 170% (w\/w) in NaCl solution at 37\u2009\u00b0C. Besides that, the limit swelling strain was less than 30%, as obtained by mechanical spectroscopy measurements in the same conditions. The viscoelastic properties of the scaffolds were also evaluated by both creep and dynamic mechanical tests. By means of using short\u2010term MEM extraction test, both types of structures (fibers and scaffolds) were found to be non\u2010cytotoxic to fibroblasts. Furthermore, osteoblasts directly cultured over chitosan fiber mesh scaffolds presented good morphology and no inhibition of cell proliferation could be observed.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:caption><jats:p>Osteoblast\u2010like cells proliferating over chitosan based fibers after 7 d of culture.<\/jats:p><\/jats:caption><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><jats:caption><jats:p>Osteoblast\u2010like cells proliferating over chitosan based fibers after 7 d of culture.<\/jats:p><\/jats:caption><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.200300100","type":"journal-article","created":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T23:05:52Z","timestamp":1092092752000},"page":"811-819","source":"Crossref","is-referenced-by-count":211,"title":["Production and Characterization of Chitosan Fibers and 3\u2010D Fiber Mesh Scaffolds for Tissue Engineering Applications"],"prefix":"10.1002","volume":"4","author":[{"given":"Kadriye","family":"Tuzlakoglu","sequence":"first","affiliation":[]},{"given":"Catarina M.","family":"Alves","sequence":"additional","affiliation":[]},{"given":"Joao F.","family":"Mano","sequence":"additional","affiliation":[]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,8,9]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-5148(00)00038-9"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(01)00691-3"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6700(94)90030-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00127-7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(97)00309-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00299-4"},{"key":"e_1_2_6_7_2","first-page":"1","volume":"4461","author":"Denkbas E. 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