{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T08:52:39Z","timestamp":1768035159873,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T00:00:00Z","timestamp":1278028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T00:00:00Z","timestamp":1278028800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2010,7,2]],"date-time":"2010-07-02T00:00:00Z","timestamp":1278028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Portuguese Foundation for Science and Technology through funds from POCTI and\/or FEDER"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2010,10,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The applicability of CMCht\/PAMAM dendrimer nanoparticles for CNS applications was investigated. AFM and TEM observations revealed that the nanoparticles possessed a nanosphere\u2010like shape with a size from 22.0 to 30.7\u2009nm. The nanoparticles could be bound to fluorescent\u2010probe FITC for tracing purposes. Post\u2010natal hippocampal neurons and cortical glial cells were both able to internalize the FITC\u2010labeled CMCht\/PAMAM dendrimer nanoparticles with high efficiency. The percentage of positive cells internalizing the nanoparticles varied, reaching a peak after 48\u2009h of incubation. Further experiments for periods up to 7\u2009d revealed that the periodical addition of FITC\u2010labelled CMCht\/PAMAM dendrimer nanoparticles was needed to maintain the overall percentage of cells internalizing them. Finally, it was also observed that cell viability was not significantly affected by the incubation of dendrimer nanoparticles.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.201000005","type":"journal-article","created":{"date-parts":[[2010,7,6]],"date-time":"2010-07-06T21:56:24Z","timestamp":1278453384000},"page":"1130-1140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Carboxymethylchitosan\/Poly(amidoamine) Dendrimer Nanoparticles in Central Nervous Systems\u2010Regenerative Medicine: Effects on Neuron\/Glial Cell Viability and Internalization Efficiency"],"prefix":"10.1002","volume":"10","author":[{"given":"Ant\u00f3nio J.","family":"Salgado","sequence":"first","affiliation":[]},{"given":"Joaquim M.","family":"Oliveira","sequence":"additional","affiliation":[]},{"given":"Rog\u00e9rio P.","family":"Pirraco","sequence":"additional","affiliation":[]},{"given":"Vitor H.","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"Joana S.","family":"Fraga","sequence":"additional","affiliation":[]},{"given":"Alexandra P.","family":"Marques","sequence":"additional","affiliation":[]},{"given":"Nuno M.","family":"Neves","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o F.","family":"Mano","sequence":"additional","affiliation":[]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Sousa","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2010,7,2]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2003.07.007"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20706"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.10.051"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/157341309789378014"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2685"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2009.05.002"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425240902939143"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200600076"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2004.06.009"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4095(200006)12:11<843::AID-ADMA843>3.0.CO;2-T"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2007.06.007"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00023-J"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012098005098"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018983904537"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022604120952"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221450098"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303403322168019"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2003.12.013"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302516"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2007.05.022"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.08.004"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2008.07.005"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.04.026"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.05.011"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308615200"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0325"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1171"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(04)03276-3"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(99)00246-1"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200800165"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01893"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1177\/0883911509104081"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.3727\/095535491820873191"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008901612674"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201000005","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201000005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.201000005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T03:25:29Z","timestamp":1697513129000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.201000005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,2]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10,8]]}},"alternative-id":["10.1002\/mabi.201000005"],"URL":"https:\/\/doi.org\/10.1002\/mabi.201000005","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,2]]}}}