{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T10:37:54Z","timestamp":1768732674342,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,8,26]],"date-time":"2010-08-26T00:00:00Z","timestamp":1282780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2010,11,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Electrospun scaffolds are widely used for various biomedical applications. In this study, we prepared electrospun bioactive composite scaffolds combining hydroxyapatite, collagen (Col) and a synthetic polymer\u2014PolyActive\u2122\u2014to mimic naturally occurring extracellular matrix for in situ bone regeneration. Human mesenchymal stem cells (hMSCs) adhered and proliferated on these scaffolds. Cells on all scaffold types showed an increased metabolic activity with time. On day 4, the metabolic activity of cells cultured on PolyActive\u2122 (PA)\u2013hydroxyapatite (HA)\u2013Col in 1,1,1,3,3,3\u2010hexafluoro\u20102\u2010propanolhexafluoro\u20102\u2010propanol (HFIP) was significantly higher than that of cells grown on PA\u2013Col samples. Furthermore, on day 6, cells on PA\u2013HA\u2013Col in HFIP showed significantly higher metabolic activity than those on PA and PA\u2013Col scaffolds. Quantitative PCR analysis for a panel of osteogenic genes showed statistically significant differences between scaffolds. Cells cultured on PA\u2013HA scaffolds had a significantly higher osteonectin and RunX2 expression compared to those on PA\u2013HA\u2013Col scaffolds. Cells on PA\u2013HA\u2013Col in HFIP scaffolds had significantly higher expression of alkaline phosphatase (ALP) and Col 1 compared to PA and PA\u2013Col scaffolds respectively. The bone morphogenetic protein\u20102 and S100A4 expression of PA\u2013Col and PA\u2013HA\u2013Col constructs was significantly lower than the basal level expression of cells on PA scaffolds. Although not statistically significant in all cases, cells cultured on PA\u2013HA\u2013Col in HFIP and PA\u2013HA scaffolds had the highest expression for most of the genes analysed. The results of the study demonstrate that bioactive composite scaffolds prepared by electrospinning could find potential use in bone regeneration applications.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/jpeg\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mgra001.jpg\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1002\/mabi.201000145","type":"journal-article","created":{"date-parts":[[2010,8,30]],"date-time":"2010-08-30T01:07:57Z","timestamp":1283130477000},"page":"1365-1373","source":"Crossref","is-referenced-by-count":55,"title":["Fabrication of Bioactive Composite Scaffolds by Electrospinning for Bone 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