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Several tissue engineering (TE)\u2010based approaches have been investigated for repairing this condition. Poly (3\u2010hydroxybutyrate\u2010<jats:italic>co<\/jats:italic>\u20103\u2010hydroxyvalerate) (PHB\u2010HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB\u2010HV\u2010based 3D scaffolds, produced by freeze\u2010drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB\u2010HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS\u2010derived cells and mesenchymal\u2010like stem cells from different sources. Finally, histocompatibility studies show that PHB\u2010HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB\u2010HV scaffolds may be suitable for SCI treatment.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/mabi201300178-gra-0001.png\" xlink:title=\"mabi201300178-gra-0001\"\/><\/jats:p><\/jats:sec>","DOI":"10.1002\/mabi.201300178","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T11:25:08Z","timestamp":1377170708000},"page":"1576-1592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Development and Characterization of a <scp>PHB<\/scp>\u2010<scp>HV<\/scp>\u2010based 3<scp>D<\/scp> Scaffold for a Tissue Engineering and Cell\u2010therapy Combinatorial Approach for Spinal Cord Injury Regeneration"],"prefix":"10.1002","volume":"13","author":[{"given":"Silvina","family":"Ribeiro\u2010Samy","sequence":"first","affiliation":[{"name":"3B's Research Group \u2013 Biomaterials, Biodegradables and 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