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In this work, instead of treating pre\u2010established mucoid biofilms, alginate lyase is immobilized onto a surface as a preventive measure against <jats:italic>P. aeruginosa<\/jats:italic> adhesion. A polydopamine dip\u2010coating strategy is employed for functionalization of polycarbonate surfaces. Enzyme immobilization is confirmed by surface characterization. Surfaces functionalized with alginate lyase exhibit anti\u2010adhesive properties, inhibiting the attachment of the mucoid strain. Moreover, surfaces modified with this enzyme also inhibit the adhesion of the tested non\u2010mucoid strain. Unexpectedly, treatment with heat\u2010inactivated enzyme also inhibits the attachment of mucoid and non\u2010mucoid <jats:italic>P. aeruginosa<\/jats:italic> strains. These findings suggest that the antibacterial performance of alginate lyase functional coatings is catalysis\u2010independent, highlighting the importance of further studies to better understand its mechanism of action against <jats:italic>P. aeruginosa<\/jats:italic> strains.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mabi201600077-abs-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.201600077","type":"journal-article","created":{"date-parts":[[2016,5,21]],"date-time":"2016-05-21T21:14:56Z","timestamp":1463865296000},"page":"1301-1310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Polydopamine\u2010Mediated Immobilization of Alginate Lyase to Prevent <i>P. aeruginosa<\/i> Adhesion"],"prefix":"10.1002","volume":"16","author":[{"given":"Diana","family":"Alves","sequence":"first","affiliation":[{"name":"CEB \u2013 Centre of Biological Engineering LIBRO \u2013 Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira University of Minho  Campus de Gualtar 4710\u2010057 Braga Portugal"}]},{"given":"Tadas","family":"Sileika","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Department Northwestern University  2145 Sheridan Road Evanston IL 60208 USA"}]},{"given":"Phillip B.","family":"Messersmith","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Department Northwestern University  2145 Sheridan Road Evanston IL 60208 USA"},{"name":"Department of Bioengineering and Department of Materials Science and Engineering University of California  Berkeley CA 94720\u20101760 USA"}]},{"given":"Maria Ol\u00edvia","family":"Pereira","sequence":"additional","affiliation":[{"name":"CEB \u2013 Centre of Biological Engineering LIBRO \u2013 Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira University of Minho  Campus de Gualtar 4710\u2010057 Braga Portugal"}]}],"member":"311","published-online":{"date-parts":[[2016,5,19]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016175502756"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21838"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_2_6_4_1","first-page":"204","volume":"82","author":"del Pozo J. 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