{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:33:23Z","timestamp":1761676403336},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T00:00:00Z","timestamp":1466985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p>Microbial colonization of indwelling devices remains a major concern in modern healthcare. Developing approaches to prevent biomaterial\u2010associated infections (BAI) is, therefore, in great demand. This study aimed to immobilize two antimicrobial peptides (polymyxins B and E) onto polydimethylsiloxane (PDMS) using two polydopamine (pDA)\u2010based approaches: the conventional two\u2010step method involving the deposition of a pDA layer to which biomolecules are immobilized, and a one\u2010step method where peptides are dissolved together with dopamine before its polymerization. Surface characterization confirms the immobilization of polymyxins onto PDMS at a non\u2010toxic concentration. Immobilization of polymyxins using a one\u2010step pDA\u2010based approach is able to prevent <jats:italic>Pseudomonas aeruginosa<\/jats:italic> adhesion and kill a significant fraction of the adherent ones. Living cells adhered to these modified surfaces exhibit the same susceptibility pattern as cells adhered to unmodified surfaces, highlighting no resistance development. Results suggest that polymyxins immobilization holds a great potential as an additional antimicrobial functionality in the design of biomaterials.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mabi201600122-abs-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p>","DOI":"10.1002\/mabi.201600122","type":"journal-article","created":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T10:16:43Z","timestamp":1467022603000},"page":"1450-1460","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Bio\u2010Inspired Coating Strategies for the Immobilization of Polymyxins to Generate Contact\u2010Killing Surfaces"],"prefix":"10.1002","volume":"16","author":[{"given":"Diana","family":"Alves","sequence":"first","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering LIBRO\u2014Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira University of Minho Campus de Gualtar  4710\u2010057 Braga Portugal"}]},{"given":"Maria Ol\u00edvia","family":"Pereira","sequence":"additional","affiliation":[{"name":"CEB\u2014Centre of Biological Engineering LIBRO\u2014Laborat\u00f3rio de Investiga\u00e7\u00e3o em Biofilmes Ros\u00e1rio Oliveira University of Minho Campus de Gualtar  4710\u2010057 Braga Portugal"}]}],"member":"311","published-online":{"date-parts":[[2016,6,27]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004528"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.07.048"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro821"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.clpt.6100247"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02469.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.09.010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.5853-5859.1996"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-75418-3_9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2013.05.003"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2014.0169"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201301686"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reactfunctpolym.2015.08.010"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.01.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.07.089"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2013.01.017"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.11.005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2014.889120"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl181"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/326503"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1086\/429323"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2007.12.003"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2010.05.002"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00922-10"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01576-06"},{"key":"e_1_2_6_27_1","first-page":"84","volume-title":"Science against Microbial Pathogens: Communicating Current Research and Technological Advances","author":"Green D.","year":"2011"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.02.028"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30673"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201200177"},{"key":"e_1_2_6_31_1","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically","year":"2003"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-0294(97)80099-4"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1346\/CCMN.1995.0430411"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm4008343"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.001"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.02840.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.23342"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2010.515027"},{"key":"e_1_2_6_39_1","first-page":"1","volume":"2015","author":"Yu Z.","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.5.5.811"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit453"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70850-3"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.12.015"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1JM13994K"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1147241"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113087"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2012.0313"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201201156"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2014.06.027"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-8686(97)00040-7"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569821"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.10069"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3629258"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.04.010"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4172-4179.2002"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.201600122","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.201600122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:11:19Z","timestamp":1696482679000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.201600122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,27]]},"references-count":55,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1002\/mabi.201600122"],"URL":"https:\/\/doi.org\/10.1002\/mabi.201600122","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,27]]}}}